Document Detail


Expression of nerve growth factor (NGF) isoforms in the rat uterus during pregnancy: accumulation of precursor proNGF.
MedLine Citation:
PMID:  15637294     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mechanisms that promote the transient degenerative changes in the uterus innervation during pregnancy remain incompletely understood. Signaling by the nerve growth factor (NGF)-beta is important for maintaining the density of peripheral sympathetic innervation. Here, we analyzed the spatial and temporal expression of NGF isoforms in the rat uterus using RT-PCR, immunoblot analysis, and immunohistochemistry during pregnancy (d 7, 14, and 21), and postpartum (d 1, 8, and 22). Western blot analysis using antibodies to mature NGF-beta and to proNGF domain demonstrated a significant decrease in mature NGF-beta at gestational d 14 and 21 (term pregnancy) and 1 d postpartum, which paralleled a remarkable accumulation of the 26-28-, 32-, and 60-kDa proNGF forms. There were diminished ratios of mature NGF-beta to proNGF independent of uterus growth on the same gestational days. Immunohistochemistry revealed a progressive NGF-beta decline throughout pregnancy in the myometrium and a near absence at term pregnancy, which contrasted with increased NGF immunostaining in the intermyometrial connective tissue layers. More importantly, proNGF-specific antibodies identified the increased NGF immunoreactivity in the intermyometrial layers at term pregnancy as proNGF and not mature NGF-beta. Alterations in the processing of NGF and accumulation of proNGF in the intermyometrial layers, where axonal degeneration occurs, may contribute significantly to the pregnancy-related uterine denervation and to the control of myometrial activity.
Authors:
Edgar Lobos; Claudia Gebhardt; Annett Kluge; Katharina Spanel-Borowski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-01-06
Journal Detail:
Title:  Endocrinology     Volume:  146     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-16     Completed Date:  2005-04-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1922-9     Citation Subset:  AIM; IM    
Affiliation:
Institute of Anatomy, University of Leipzig, Liebigstrasse 13, 04103 Leipzig, Germany. Edgar.Lobos@medizin.uni-leipzig.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Female
Immunohistochemistry
Nerve Growth Factor / analysis*,  genetics,  immunology
Postpartum Period / metabolism
Pregnancy
Pregnancy, Animal / metabolism*
Protein Isoforms
Protein Precursors / analysis,  metabolism*
RNA, Messenger / analysis
Rats
Rats, Inbred Lew
Reverse Transcriptase Polymerase Chain Reaction
Uterus / chemistry*,  metabolism
Chemical
Reg. No./Substance:
0/Protein Isoforms; 0/Protein Precursors; 0/RNA, Messenger; 9061-61-4/Nerve Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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