| Expression of multiple heparin-binding growth factor species by murine embryonal carcinoma and embryonic stem cells. | |
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MedLine Citation:
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PMID: 2627890 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Culture medium conditioned by P19 embryonal carcinoma (EC) cells contains potent mitogenic activity which is markedly potentiated when the medium is conditioned in the presence of heparin. Fractionation of P19 medium conditioned in the presence of heparin reveals the existence of two biochemically distinct growth factor species both of which exhibit high affinity for immobilised heparin and significant activity as amphibian mesoderm-inducing agents. One of the species is recovered as a single polypeptide of apparent Mr = 15,000. This molecule is immunologically related to the protein product of the human K-FGF proto-oncogene. Transcripts derived from the murine K-FGF gene are also expressed by both differentiated and undifferentiated EC cells and embryonic stem cells. The second heparin-binding growth factor is recovered as a complex of four polypeptides, the largest of which has an apparent Mr = 17,000. This agent is immunologically and biochemically distinct from both acidic and basic fibroblast growth factor as well as K-FGF, and represents the predominant mitogenic activity in EC-cell-conditioned medium. |
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Authors:
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J K Heath; G D Paterno; A C Lindon; D R Edwards |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 107 ISSN: 0950-1991 ISO Abbreviation: Development Publication Date: 1989 Sep |
Date Detail:
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Created Date: 1990-04-16 Completed Date: 1990-04-16 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 113-22 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Oxford, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography Embryonal Carcinoma Stem Cells Fibroblast Growth Factors / genetics Gene Expression Growth Substances / analysis, genetics* Heparin / analysis, genetics* Immunoblotting Mice Neoplastic Stem Cells / metabolism* Restriction Mapping |
| Chemical | |
Reg. No./Substance:
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0/Growth Substances; 62031-54-3/Fibroblast Growth Factors; 9005-49-6/Heparin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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