Document Detail


Expression of mucin (MUC) genes in mucoepidermoid carcinoma.
MedLine Citation:
PMID:  21072847     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES/HYPOTHESIS: Mucoepidermoid carcinoma (MEC) is the most common malignant salivary gland tumor. The presence of mucin (MUC) genes has been correlated with patient prognosis using immunohistochemical techniques. This study was undertaken to 1) investigate the expression of newly discovered MUC genes in MEC specimens, 2) assess the correlation of this expression to prognosis, and 3) further investigate the correlation of previously identified MUC genes to prognosis using molecular techniques.
STUDY DESIGN: Retrospective chart review and sample isolation.
METHODS: Twenty-three patients with a diagnosis of MEC underwent mucin gene analysis of tumor and normal surrounding salivary gland tissue. Initial expression analysis of recently identified mucin genes MUC 12, 13, 17, 18, and 19 was performed using reverse transcription-polymerase chain reaction (RT-PCR) followed by quantitative assessment with quantitative PCR. Additional assessments of mucin genes MUC 1 and MUC 4, which have previously been associated with MEC, were also analyzed with real-time RT-PCR.
RESULTS: MEC was associated with MUC 19 expression in 65% of tumor samples compared to only 26% of normal tissue (P = .02). MUC 18 demonstrated equal expression in both tumor and normal tissue. MUC 12 and 17 were not expressed in either MEC or normal salivary gland. MUC 13 was found in 13% of tumors and 0% of normal samples. MUC 1 and MUC 4 were expressed 4.2- and 21-fold higher in stage I disease in tumor tissue compared to normal, respectively.
CONCLUSIONS: Presence of MUC 19 is suggestive of mucoepidermoid carcinoma, and higher expression of MUC 1 and MUC 4 is correlated with earlier stage disease.
Authors:
Nima Shemirani; Vladimir Osipov; Alex Kolker; Pawjai Khampang; Joseph E Kerschner
Related Documents :
19586997 - Detection and quantification of five major periodontal pathogens by single copy gene-ba...
16888767 - Effects of global system for mobile communications 1800 mhz radiofrequency electromagne...
19413767 - Detection of metallo-beta-lactamases-encoding genes in environmental isolates of aeromo...
23628997 - Magnesium deficiency phenotypes upon multiple knockout of arabidopsis thaliana mrs2 cla...
15342597 - A novel mutation within the central listeria monocytogenes regulator prfa that results ...
9109497 - Oncogene- and tumor-suppressor gene-related proteins in plants and fungi.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Laryngoscope     Volume:  121     ISSN:  1531-4995     ISO Abbreviation:  Laryngoscope     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-24     Completed Date:  2011-01-25     Revised Date:  2014-11-13    
Medline Journal Info:
Nlm Unique ID:  8607378     Medline TA:  Laryngoscope     Country:  United States    
Other Details:
Languages:  eng     Pagination:  167-70     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Carcinoma, Mucoepidermoid / diagnosis,  genetics*,  metabolism
Gene Expression
Humans
Mucin-1 / genetics,  metabolism
Mucin-4 / genetics,  metabolism
Mucins / genetics*,  metabolism
Prognosis
Reverse Transcriptase Polymerase Chain Reaction
Salivary Gland Neoplasms / diagnosis,  genetics*,  metabolism
Salivary Glands / metabolism
Tumor Markers, Biological / analysis
Grant Support
ID/Acronym/Agency:
DC007903/DC/NIDCD NIH HHS; R01 DC007903/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
0/MUC13 protein, human; 0/Mucin-1; 0/Mucin-4; 0/Mucins; 0/Tumor Markers, Biological

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Safety of intranasal Bevacizumab (avastin) treatment in patients with hereditary hemorrhagic telangi...
Next Document:  Skew deviation after intratympanic gentamicin therapy.