Document Detail


Expression of monocyte chemoattractant protein-1 in brain tissue of patients with intractable epilepsy.
MedLine Citation:
PMID:  18402383     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the expression ofmonocyte chemoattractant protein-1 (MCP-1) in the brain tissue of patients with intractable epilepsy (IE) and to explore its role in the pathogenesis of IE. MATERIAL: Patients with IE were collected from the Department of Neurosurgery of several Chinese hospitals between 2002 and 2005. The average age of these 40 patients was 23.65 +/- 10.14 (X +/- SD) years (11 - 58 years), with 19 males and 21 females. In addition to the typical symptoms and distinct EEG features of epilepsy, all recruited patients met the requirements of intractable epilepsy. METHODS: On the basis of positive results obtained from gene chip analysis, the expression of MCP-1 was first investigated in the brain tissue of IE patients (40 cases) using Western Blotting and immunohistochemistry and then compared with the controls. RESULTS: Gene chip scanning demonstrated that expression of MCP-1 mRNA was upregulated in the brain tissue of patients with IE. Western Blotting and immunohistochemical experiments further confirmed that, as compared with that in the control group, expression of MCP-1 protein was significantly increased in the IE patients (p < 0.05). CONCLUSION: These results suggest that MCP-1 are involved in the pathogenesis of IE.
Authors:
Y Wu; X Wang; X Mo; Z Xi; F Xiao; J Li; X Zhu; G Luan; Y Wang; Y Li; J Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical neuropathology     Volume:  27     ISSN:  0722-5091     ISO Abbreviation:  Clin. Neuropathol.     Publication Date:    2008 Mar-Apr
Date Detail:
Created Date:  2008-04-11     Completed Date:  2008-05-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8214420     Medline TA:  Clin Neuropathol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  55-63     Citation Subset:  IM    
Affiliation:
Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Blotting, Western
Brain / metabolism*
Chemokine CCL2 / biosynthesis*
Child
Epilepsy / metabolism*
Female
Gene Expression
Gene Expression Profiling
Humans
Immunohistochemistry
Male
Middle Aged
Oligonucleotide Array Sequence Analysis
RNA, Messenger / analysis
Up-Regulation
Chemical
Reg. No./Substance:
0/CCL2 protein, human; 0/Chemokine CCL2; 0/RNA, Messenger

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