Document Detail


Expression of the monocarboxylate transporter 1 (MCT1) in cells of the porcine intestine.
MedLine Citation:
PMID:  18313333     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Uptake of energy into cells and its allocation to individual cellular compartments by transporters are essential for tissue homeostasis. The present study gives an analysis of MCT1 expression and its cellular occurrence in the porcine intestine. Tissue portions from duodenum, jejunum, ileum, colon ascendens, colon transversum and colon descendens were collected and prepared for immunohistochemistry, Western blot and real time RT-PCR. A 169bp porcine MCT1 cDNA fragment was amplified and published. MCT1 mRNA expression in the large intestine was 20 fold higher compared to the small intestine. Western blot detected a single protein band of 41kDa at a much higher amount of MCT1 protein in the large intestine vs. the small intestine. MCT1 protein was detected in mitochondrial fractions of the large but not the small intestine. Immunohistochemistry in the small intestine showed that immune cells in the lamina propria and in the lymphoid follicles primarily expressed MCT1 while in the colon epithelial cells were the main source of MCT1. In summary, cellular expression of MCT1 differs between epithelial cells in the colon and small intestine. A possible role of MCT1 for uptake of butyrate into immune cells and the overall role of MCT1 for intestinal immune cell function remains elusive.
Authors:
Harald Welter; Rolf Claus
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Publication Detail:
Type:  Journal Article     Date:  2008-01-25
Journal Detail:
Title:  Cell biology international     Volume:  32     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-19     Completed Date:  2008-07-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  638-45     Citation Subset:  IM    
Affiliation:
Institute for Animal Husbandry and Animal Breeding, University of Hohenheim, Garbenstrasse 17, D-70599 Stuttgart, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Western
Gene Expression
Immunohistochemistry
Intestines / metabolism*
Male
Molecular Sequence Data
Monocarboxylic Acid Transporters / chemistry,  genetics,  metabolism*
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Swine / metabolism
Symporters / chemistry,  genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Monocarboxylic Acid Transporters; 0/RNA, Messenger; 0/Symporters; 0/monocarboxylate transport protein 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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