| Expression of miR-21, miR-31, miR-96 and miR-135b is correlated with the clinical parameters of colorectal cancer. | |
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MedLine Citation:
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PMID: 22844381 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The aim of this study was to determine the expression of miR-21, miR-31, miR-96 and miR-135b in 52 paired colorectal cancer (CRC) tissues and to analyze the correlation between microRNAs (miRNAs) and clinicopathological features. We developed a quantification method that relies on a standard plot, constructed from known concentrations of standards, in order to measure the number of miRNAs. In addition to this, we analyzed the expression levels of miR-21, miR-31, miR-96 and miR-135b in 52 cases of primary CRC and corresponding normal mucosal tissue using real-time PCR with SYBR-Green I. An independent sample t-test was used to compare the differential expression between tumor tissues and normal mucosal tissues. The Mann-Whitney U and Kruskall-Wallis tests were used to compare the correlation between miRNA expression levels and clinicopathological features. The expression of miR-21, miR-31, miR-96 and miR-135b was upregulated in the CRC tissues compared to normal mucosal tissues (P<0.05). Furthermore, miR-21 and miR-135b were positively correlated with the clinical stage (P=0.048 and P=0.029, respectively), while miR-96 and miR-135b were correlated with liver metastasis (P=0.006 and P=0.013, respectively). Our results suggest that miR-21, miR-31, miR-96 and miR-135b may function in the process of CRC development and progression. miR-135b levels in particular may correlate with the degree of malignancy. |
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Authors:
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Xin Min Xu; Jian Chang Qian; Zhou Lu Deng; Zhe Cai; Tao Tang; Peng Wang; Ke Hua Zhang; Jian-Ping Cai |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-14 |
Journal Detail:
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Title: Oncology letters Volume: 4 ISSN: 1792-1082 ISO Abbreviation: - Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-7-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101531236 Medline TA: Oncol Lett Country: - |
Other Details:
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Languages: ENG Pagination: 339-345 Citation Subset: - |
Affiliation:
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Graduate School, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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