Document Detail


Expression of membrane and nuclear progesterone receptors in two human placental choriocarcinoma cell lines (JEG-3 and BeWo): Effects of syncytialization.
MedLine Citation:
PMID:  21455559     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A vital function of the human placenta is to produce steroid hormones such as progesterone, which are essential for the maintenance of pregnancy and the onset of parturition. Although choriocarcinoma cell lines are valuable placental models for investigations of steroid hormone actions, little is known about the expression of progesterone receptors (PRs) in these cell lines. Therefore, in this study, the expression of membrane and nuclear PRs was investigated in cultures of fusigenic (BeWo) and non-fusigenic (JEG-3) human choriocarcinoma cell lines. In addition, the effects of an inducer of syncytialization (forskolin) on the PR expression in BeWo cells were assessed. Quantitative RT-PCR revealed that in fully syncytialized BeWo cells (treated with 50 µM forskolin for 72 h) there was a significant down-regulation of mPRα and up-regulation of mPRβ and of the progesterone membrane component-1 (PGRMC1) when compared with non-syncytialized BeWo cells. Expression of all the mPR and PGRMC1 mRNAs was significantly lower in JEG-3 cells compared to non-syncytialized BeWo cells. Interestingly, expression of PR-B was unaltered between the two BeWo states but was significantly higher in JEG-3 cells. Immunofluorescence analysis revealed that mPR proteins are differentially expressed in these choriocarcinoma cell lines as well as in the human placenta. The data demonstrate that human choriocarcinoma cell lines have a complex system of progesterone signalling involving multiple classes of PRs. The finding that syncytialization is accompanied by changes in the expression of these receptors may suggest that this process influences progesterone signalling.
Authors:
Elena Zachariades; Helen Foster; Anastasia Goumenou; Peter Thomas; Mariann Rand-Weaver; Emmanouil Karteris
Related Documents :
25289099 - Microrna-7 sensitizes non-small cell lung cancer cells to paclitaxel.
25156829 - Glucocerebrosidase depletion enhances cell-to-cell transmission of α-synuclein.
25359669 - A new epithelial cell line, hbf from caudal fin of endangered yellow catfish, horabagru...
25460309 - Profiling of concanavalin a-binding glycoproteins in human hepatic stellate cells activ...
21400689 - High-throughput lectin microarray-based analysis of live cell surface glycosylation.
25370579 - Epigallocatechin‑3‑gallate induces the apoptosis of hepatocellular carcinoma lm6 ce...
2556479 - Il-2 protects t lymphocytes from glucocorticoid-induced dna fragmentation and cell death.
16425399 - Msx2 overexpression inhibits gemcitabine-induced caspase-3 activity in pancreatic cance...
16876159 - Alterations in hippocampal neurogenesis following traumatic brain injury in mice.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-23
Journal Detail:
Title:  International journal of molecular medicine     Volume:  -     ISSN:  1791-244X     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-4-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Centre for Cell Chromosome Biology, Biosciences, School of Health Sciences and Social Care, Brunel University, Uxbridge, UB8 3PH, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Flagellin promotes the proliferation of gastric cancer cells via the Toll-like receptor 5.
Next Document:  Cocktail therapy with a combination of interferon, ribavirin and angiotensin-II type 1 receptor bloc...