Document Detail

Expression of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-1 in hepatic stellate cells during rat hepatic fibrosis and its intervention by IL-10.
MedLine Citation:
PMID:  15793858     Owner:  NLM     Status:  MEDLINE    
AIM: To investigate the expression of matrix metallopr-oteinase-2 and tissue inhibitor of metalloproteinase-1 in hepatic fibrosis and the antifibrogenic role of exogenous interleukin-10 (IL-10). METHODS: Hepatic fibrosis was induced by CCl(4) administration and 60 male Sprague-Dawley rats were randomly divided into normal control group (group N, 8 rats), CCl(4)-induced group (group C, 28 rats) and IL-10-treated group (group I, 24 rats). At the beginning of the 7(th) and 11(th) wk, rats in each group were routinely perfused with pronase E and type IV collagenase through portal vein catheter and the suspension was centrifuged by 11% Nycodenz density gradient to isolate hepatic stellate cells (HSCs). RT-PCR was used to analyze mRNA of MMP-2 and TIMP-1 from freshly isolated cells. Densitometric data were standardized with beta-actin signals. Immunocytochemistry was performed to detect MMP-2 and TIMP-1 expression in HSC cultured for 72 h. RESULTS: Compared to group N in the 7(th) wk, MMP-2 and TIMP-1 mRNA increased in group C (P = 0.001/0.001) and group I (P = 0.001/0.009). The level of MMP-2 and TIMP-1 mRNA in group I was significantly lower than that in group C (P = 0.001/0.001). In the 11(th) wk, MMP-2 mRNA in group I was still lower than that in group C (P = 0.005), but both dropped compared with that in the 7(th) week (P = 0.001/0.004). TIMP-1 mRNA in group I was still lower than that in group C (P = 0.001), and increased in group C (P = 0.001) while decreased in group I (P = 0.042) compared with that in the 7(th) wk. Same results were found by immunocytochemistry. CONCLUSION: Expression of MMP-2 and TIMP-1 is increased in hepatic fibrosis. IL-10 exhibits an antifibrogenic effect by suppressing MMP-2 and TIMP-1 expression.
Wei-Da Zheng; Li-Juan Zhang; Mei-Na Shi; Zhi-Xin Chen; Yun-Xin Chen; Yue-Hong Huang; Xiao-Zhong Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  11     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-28     Completed Date:  2005-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  1753-8     Citation Subset:  IM    
Department of Gastroenterology, Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China.
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MeSH Terms
Carbon Tetrachloride
Interleukin-10 / pharmacology*
Liver / metabolism,  pathology
Liver Cirrhosis / drug therapy*,  pathology,  physiopathology*
Matrix Metalloproteinase 2 / genetics*,  metabolism
RNA, Messenger / analysis
Rats, Sprague-Dawley
Tissue Inhibitor of Metalloproteinase-1 / genetics*,  metabolism
Reg. No./Substance:
0/RNA, Messenger; 0/Tissue Inhibitor of Metalloproteinase-1; 130068-27-8/Interleukin-10; 56-23-5/Carbon Tetrachloride; EC Metalloproteinase 2

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