Document Detail

Expression of matrix metalloproteinase-2 and -9 and of tissue inhibitor of matrix metalloproteinase-1 in liver regeneration from oval cells in rat.
MedLine Citation:
PMID:  18678246     Owner:  NLM     Status:  MEDLINE    
Oval cells participate in liver regeneration when hepatocyte replication is impaired. These precursor cells proliferate in periportal regions and organize in ductules. They are surrounded by a basement membrane, the degradation of which by matrix metalloproteinases (MMP) might trigger their terminal differentiation into hepatocytes. We studied the expression of MMP-2 and MMP-9 and that of one of their tissue inhibitors (TIMP-1) in a model of hepatic regeneration from precursor cells. Regeneration was induced by treating rats with 2-acetylaminofluorene followed by partial hepatectomy. MMP-2 and MMP-9 hepatic expression paralleled oval cell number with a peak at day 9-14 after hepatectomy. They were mainly detected in oval cells. TIMP-1 mRNA and oncostatin M receptor mRNA, a major regulator of TIMP-1 synthesis, markedly increased from day 1 after surgery until day 9 and then declined; they were mainly detected in interlobular bile duct cells and oval cells until day 14. In agreement with the in vivo data, the WB-F344 liver precursor cell line expressed MMP-2 and MMP-9, as well as TIMP-1 and oncostatin M receptor. These data suggest that (a) early increased TIMP-1 synthesis by biliary and oval cells favors basement membrane deposition around proliferating ductular structures through MMP inhibition, (b) delayed increased MMP expression, concomitant to decreased TIMP-1 synthesis, leads to basement membrane degradation, preceding oval cell differentiation, (c) the oncostatin M pathway might play a major role in increased TIMP-1 synthesis.
T Pham Van; D Couchie; N Martin-Garcia; Y Laperche; E S Zafrani; P Mavier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-16
Journal Detail:
Title:  Matrix biology : journal of the International Society for Matrix Biology     Volume:  27     ISSN:  0945-053X     ISO Abbreviation:  Matrix Biol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-12-01     Completed Date:  2009-06-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9432592     Medline TA:  Matrix Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  674-81     Citation Subset:  IM    
INSERM U841, Université Paris 12, AP-HP, Groupe Hospitalier Henri Mondor-Albert Chenevier, Département de Pathologie, Créteil, F-94000 France.
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MeSH Terms
Cells, Cultured
Gene Expression Regulation, Enzymologic*
Hepatocytes / enzymology
In Situ Hybridization
Liver / cytology*,  enzymology*
Liver Regeneration* / genetics
Matrix Metalloproteinase 2 / genetics,  metabolism*
Matrix Metalloproteinase 9 / genetics,  metabolism*
Oncostatin M / genetics,  metabolism
Rats, Inbred F344
Receptors, Oncostatin M / genetics,  metabolism
Tissue Inhibitor of Metalloproteinase-1 / genetics,  metabolism*
Reg. No./Substance:
0/Receptors, Oncostatin M; 0/Tissue Inhibitor of Metalloproteinase-1; 106956-32-5/Oncostatin M; EC Metalloproteinase 2; EC Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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