| Expression of matrix metalloproteinase-2 and -9 and of tissue inhibitor of matrix metalloproteinase-1 in liver regeneration from oval cells in rat. | |
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MedLine Citation:
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PMID: 18678246 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oval cells participate in liver regeneration when hepatocyte replication is impaired. These precursor cells proliferate in periportal regions and organize in ductules. They are surrounded by a basement membrane, the degradation of which by matrix metalloproteinases (MMP) might trigger their terminal differentiation into hepatocytes. We studied the expression of MMP-2 and MMP-9 and that of one of their tissue inhibitors (TIMP-1) in a model of hepatic regeneration from precursor cells. Regeneration was induced by treating rats with 2-acetylaminofluorene followed by partial hepatectomy. MMP-2 and MMP-9 hepatic expression paralleled oval cell number with a peak at day 9-14 after hepatectomy. They were mainly detected in oval cells. TIMP-1 mRNA and oncostatin M receptor mRNA, a major regulator of TIMP-1 synthesis, markedly increased from day 1 after surgery until day 9 and then declined; they were mainly detected in interlobular bile duct cells and oval cells until day 14. In agreement with the in vivo data, the WB-F344 liver precursor cell line expressed MMP-2 and MMP-9, as well as TIMP-1 and oncostatin M receptor. These data suggest that (a) early increased TIMP-1 synthesis by biliary and oval cells favors basement membrane deposition around proliferating ductular structures through MMP inhibition, (b) delayed increased MMP expression, concomitant to decreased TIMP-1 synthesis, leads to basement membrane degradation, preceding oval cell differentiation, (c) the oncostatin M pathway might play a major role in increased TIMP-1 synthesis. |
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Authors:
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T Pham Van; D Couchie; N Martin-Garcia; Y Laperche; E S Zafrani; P Mavier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-16 |
Journal Detail:
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Title: Matrix biology : journal of the International Society for Matrix Biology Volume: 27 ISSN: 0945-053X ISO Abbreviation: Matrix Biol. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-12-01 Completed Date: 2009-06-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9432592 Medline TA: Matrix Biol Country: Germany |
Other Details:
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Languages: eng Pagination: 674-81 Citation Subset: IM |
Affiliation:
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INSERM U841, Université Paris 12, AP-HP, Groupe Hospitalier Henri Mondor-Albert Chenevier, Département de Pathologie, Créteil, F-94000 France. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Gene Expression Regulation, Enzymologic* Hepatocytes / enzymology In Situ Hybridization Liver / cytology*, enzymology* Liver Regeneration* / genetics Male Matrix Metalloproteinase 2 / genetics, metabolism* Matrix Metalloproteinase 9 / genetics, metabolism* Oncostatin M / genetics, metabolism Rats Rats, Inbred F344 Receptors, Oncostatin M / genetics, metabolism Tissue Inhibitor of Metalloproteinase-1 / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Oncostatin M; 0/Tissue Inhibitor of Metalloproteinase-1; 106956-32-5/Oncostatin M; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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