Document Detail


Expression of macrophage migration inhibitory factor transcript and protein by first-trimester human trophoblasts.
MedLine Citation:
PMID:  10330084     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Macrophage migration inhibitory factor (MIF) was originally identified for its capacity to inhibit the random migration of macrophages in vitro. To date, the role of MIF as a pro-inflammatory cytokine, pituitary hormone, and counter-regulator of glucocorticoid action on the immune response is commonly recognized. Although recent studies suggest an involvement of MIF in reproduction, no data exist on the expression of this cytokine in early human pregnancy. In this study, we evaluated the presence of MIF protein and mRNA in specimens of chorionic villi from first-trimester human placenta. Tissues were obtained at 6-10 wk of gestation and analyzed by Western blotting, reverse transcription-polymerase chain reaction, and immunohistochemistry. Our results demonstrate that human villous tissue is a novel site of MIF synthesis. In addition, immunohistochemical analysis identified MIF protein in the cytotrophoblasts of both the inner layer of villi and in the trophoblastic cell islands. We speculate that in view of its proinflammatory features, MIF might play a critical role in human implantation and in early embryonic development.
Authors:
F Arcuri; M Cintorino; R Vatti; A Carducci; S Liberatori; L Paulesu
Related Documents :
11866424 - Insights of high-density lipoprotein apolipoprotein-mediated lipid efflux from cells.
12969324 - Is macrophage migration inhibitory factor a therapeutic target in systemic lupus erythe...
8280184 - Exogenous glucocorticoids increase macrophage secretion of apo e by cholesterol-indepen...
20005974 - Electronegative ldl induction of apoptosis in macrophages: involvement of nrf2.
10403484 - An intact nf-kappa b signaling pathway is required for maintenance of mature b cell sub...
21091644 - Mechanisms for anti-inflammatory effects of 1-[15(s)-hydroxyeicosapentaenoyl] lysophosp...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of reproduction     Volume:  60     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-06-15     Completed Date:  1999-06-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1299-303     Citation Subset:  IM    
Affiliation:
Institute of Pathological Anatomy and Histology, Department of Molecular Biology, Siena, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Female
Gene Expression*
Gestational Age
Humans
Immunohistochemistry
Macrophage Migration-Inhibitory Factors / analysis*,  genetics*
Pregnancy
RNA, Messenger / analysis*
Reverse Transcriptase Polymerase Chain Reaction
Trophoblasts / chemistry*
Chemical
Reg. No./Substance:
0/Macrophage Migration-Inhibitory Factors; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Sensitivity of bovine corpora lutea to prostaglandin F2alpha is dependent on progesterone, oxytocin,...
Next Document:  Expression and action of transforming growth factor beta (TGFbeta1, TGFbeta2, and TGFbeta3) during e...