Document Detail

Expression of macrophage migration inhibitory factor transcript and protein by first-trimester human trophoblasts.
MedLine Citation:
PMID:  10330084     Owner:  NLM     Status:  MEDLINE    
Macrophage migration inhibitory factor (MIF) was originally identified for its capacity to inhibit the random migration of macrophages in vitro. To date, the role of MIF as a pro-inflammatory cytokine, pituitary hormone, and counter-regulator of glucocorticoid action on the immune response is commonly recognized. Although recent studies suggest an involvement of MIF in reproduction, no data exist on the expression of this cytokine in early human pregnancy. In this study, we evaluated the presence of MIF protein and mRNA in specimens of chorionic villi from first-trimester human placenta. Tissues were obtained at 6-10 wk of gestation and analyzed by Western blotting, reverse transcription-polymerase chain reaction, and immunohistochemistry. Our results demonstrate that human villous tissue is a novel site of MIF synthesis. In addition, immunohistochemical analysis identified MIF protein in the cytotrophoblasts of both the inner layer of villi and in the trophoblastic cell islands. We speculate that in view of its proinflammatory features, MIF might play a critical role in human implantation and in early embryonic development.
F Arcuri; M Cintorino; R Vatti; A Carducci; S Liberatori; L Paulesu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of reproduction     Volume:  60     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-06-15     Completed Date:  1999-06-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1299-303     Citation Subset:  IM    
Institute of Pathological Anatomy and Histology, Department of Molecular Biology, Siena, Italy.
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MeSH Terms
Blotting, Western
Gene Expression*
Gestational Age
Macrophage Migration-Inhibitory Factors / analysis*,  genetics*
RNA, Messenger / analysis*
Reverse Transcriptase Polymerase Chain Reaction
Trophoblasts / chemistry*
Reg. No./Substance:
0/Macrophage Migration-Inhibitory Factors; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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