Document Detail

Expression of lysostaphin in HeLa cells protects from host cell killing by intracellular Staphylococcus aureus.
MedLine Citation:
PMID:  16482441     Owner:  NLM     Status:  MEDLINE    
The Staphylococcus aureus-specific cell wall endopeptidase lysostaphin was used as a model for an intracellular acting bactericidal antibiotic. HeLa cells were transfected with an expression vector directing the heterologous expression of lysostaphin in the cytoplasm. Expression, subcellular localization and enzymatic activity of lysostaphin were investigated by immunoblotting, fluorescent microscopy and agar diffusion assays. Both transiently and stably transfected HeLa cells showed a strong expression of active lysostaphin. After infection with S. aureus, the intracellular number of S. aureus and the host cell viability were determined. This staphylolytic activity resulted in a strong reduction of intracellular S. aureus in a time- and dose-dependent manner. Furthermore, host cells expressing lysostaphin became protected from S. aureus-induced cell death. Our data demonstrate the potential of intracellularly acting cell-wall active drugs or antibiotics that kill S. aureus without causing harm to the infected host cells.
Marcus Klein; Martin Krönke; Oleg Krut
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-16
Journal Detail:
Title:  Medical microbiology and immunology     Volume:  195     ISSN:  0300-8584     ISO Abbreviation:  Med. Microbiol. Immunol.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-09     Completed Date:  2007-03-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0314524     Medline TA:  Med Microbiol Immunol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  159-63     Citation Subset:  IM    
Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Universität zu Köln, Goldenfelsstrasse 21, 50935 Köln, Germany.
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MeSH Terms
Cytoplasm / drug effects,  microbiology
Hela Cells / chemistry*
Lysostaphin / metabolism,  pharmacology*
Staphylococcus aureus / drug effects*,  growth & development,  pathogenicity
Reg. No./Substance:

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