| Expression of lysostaphin in HeLa cells protects from host cell killing by intracellular Staphylococcus aureus. | |
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MedLine Citation:
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PMID: 16482441 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Staphylococcus aureus-specific cell wall endopeptidase lysostaphin was used as a model for an intracellular acting bactericidal antibiotic. HeLa cells were transfected with an expression vector directing the heterologous expression of lysostaphin in the cytoplasm. Expression, subcellular localization and enzymatic activity of lysostaphin were investigated by immunoblotting, fluorescent microscopy and agar diffusion assays. Both transiently and stably transfected HeLa cells showed a strong expression of active lysostaphin. After infection with S. aureus, the intracellular number of S. aureus and the host cell viability were determined. This staphylolytic activity resulted in a strong reduction of intracellular S. aureus in a time- and dose-dependent manner. Furthermore, host cells expressing lysostaphin became protected from S. aureus-induced cell death. Our data demonstrate the potential of intracellularly acting cell-wall active drugs or antibiotics that kill S. aureus without causing harm to the infected host cells. |
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Authors:
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Marcus Klein; Martin Krönke; Oleg Krut |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-02-16 |
Journal Detail:
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Title: Medical microbiology and immunology Volume: 195 ISSN: 0300-8584 ISO Abbreviation: Med. Microbiol. Immunol. Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-08-09 Completed Date: 2007-03-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0314524 Medline TA: Med Microbiol Immunol Country: Germany |
Other Details:
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Languages: eng Pagination: 159-63 Citation Subset: IM |
Affiliation:
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Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Universität zu Köln, Goldenfelsstrasse 21, 50935 Köln, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cytoplasm
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drug effects,
microbiology Hela Cells / chemistry* Humans Lysostaphin / metabolism, pharmacology* Staphylococcus aureus / drug effects*, growth & development, pathogenicity |
| Chemical | |
Reg. No./Substance:
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EC 3.4.24.75/Lysostaphin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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