| Expression of lysosome-associated membrane protein 1 (Lamp-1) and galectins in human keratinocytes is regulated by differentiation. | |
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MedLine Citation:
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PMID: 16710742 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lysosomes and their components are suspected to be involved in epidermal differentiation. In this study, lysosomal enzyme activities, expression of the lysosome-associated membrane protein 1 (Lamp-1) and expression of the epidermal galectins-1, -3 and -7 were investigated in human keratinocytes cultured at different cell densities (subconfluence, confluence and postconfluence) in order to induce differentiation. Detected by Western blot and immunofluorescence, Lamp-1 expression is transiently upregulated at culture confluence, but reduced at postconfluence. Northern blot analyses performed on subconfluent, confluent and post-confluent cultures of keratinocytes show that Lamp-1 mRNA expression is also upregulated at culture confluence, but downregulated at postconfluence. Measurements of lysosomal enzyme activities indicate a transient upregulation at culture confluence, whereas cathepsins B, C and L are particularly downregulated at postconfluence. Cell density and differentiation of epidermal cells also differentially regulates galectin expression in autocrine cultures. As the expression of galectin-1 mRNA is high in subconfluent cells, it is assumed to be associated with their proliferative state. On the other hand, as the mRNA levels for galectins-3 and -7 are notably upregulated at culture confluence (galectin-7) or at postconfluence (galectin-3), their expression is thought to be related to the differentiated state of keratinocytes. However, we collected evidence by confocal microscopy that galectin-3 and Lamp-1 do not colocalize in vitro in keratinocytes. Altogether, our results suggest that the upregulated Lamp-1 expression at confluence could be involved in keratinocyte differentiation, but apparently not through interaction with galectin-3. |
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Authors:
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Victoria Sarafian; Ralph Jans; Yves Poumay |
Publication Detail:
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Type: Journal Article Date: 2006-04-22 |
Journal Detail:
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Title: Archives of dermatological research Volume: 298 ISSN: 0340-3696 ISO Abbreviation: Arch. Dermatol. Res. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-05-19 Completed Date: 2007-08-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8000462 Medline TA: Arch Dermatol Res Country: Germany |
Other Details:
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Languages: eng Pagination: 73-81 Citation Subset: IM |
Affiliation:
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Department of Histologie-Embryologie, Facultés Universitaires Notre-Dame de la Paix, 61, Rue de Bruxelles, 5000, Namur, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cell Count Cell Differentiation Cells, Cultured DNA Primers / genetics Galectins / genetics*, metabolism* Gene Expression Regulation Humans Keratinocytes / cytology*, metabolism* Lysosome-Associated Membrane Glycoproteins / genetics*, metabolism* Lysosomes / metabolism RNA, Messenger / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Galectins; 0/LAMP1 protein, human; 0/Lysosome-Associated Membrane Glycoproteins; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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