Document Detail

Expression and localization of trefoil factor family genes in rat submandibular glands.
MedLine Citation:
PMID:  24588600     Owner:  NLM     Status:  Publisher    
The trefoil factor (TFF) family, which comprises TFF1, TFF2 and TFF3, plays an essential role in epithelial regeneration within the gastrointestinal tract. All three TFFs are present in human saliva; TFF3 is the predominant trefoil peptide. Little is known about the expression and tissue distribution of TFFs in rats, which are commonly used as a model system for human studies. We investigated the localization of the TFF genes that encode secretory peptides in rat submandibular glands (SMG). All three TFFs were expressed in rat SMG, although their location varied. Substantial amounts of TFF1 were detected only in the cytoplasm of epithelial cells in the SMG granular convoluted tubules (GCT), while TFF2 and TFF3 were widely distributed in the cytoplasm of epithelial cells of intercalated ducts (ID), striated ducts (SD) and interlobular ducts (ILD). The three TFFs also were detected especially in the lumens of the SD and ILD. Semi-quantitative RT-PCR and in situ hybridization experiments confirmed TFF1, TFF2 and TFF3 mRNA expressions in the SMG. Greater expression of TFF peptides and mRNA was observed in male rats than in females. The broad expression of TFFs in rat SMG cells and lumens suggests that TFFs function in this organ by their secretion into the duct lumens. We also found differences in TFF expression profiles between rat and human SMG; therefore, caution should be exercised when using rats as a model for human TFF studies.
Jf Wu; J Zhang; G Xue; Hq Zhang
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-3-3
Journal Detail:
Title:  Biotechnic & histochemistry : official publication of the Biological Stain Commission     Volume:  -     ISSN:  1473-7760     ISO Abbreviation:  Biotech Histochem     Publication Date:  2014 Mar 
Date Detail:
Created Date:  2014-3-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107378     Medline TA:  Biotech Histochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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