Document Detail

Expression and localization of transcripts of MT5-MMP and its related MMP in the ovary of the medaka fish Oryzias latipes.
MedLine Citation:
PMID:  11267666     Owner:  NLM     Status:  MEDLINE    
cDNA clones of MT5-matrix metalloproteinase (MT5-MMP) and a related protein (designated MT5-MMP-del) were isolated by screening the cDNA library and by 3'-rapid amplification of cDNA ends using an ovary RNA of the medaka fish Oryzias latipes. The MT5-MMP clone encodes a protein of 546 amino acids while the MT5-MMP-del clone encodes a protein of 431 amino acids. Compared with mammalian counterparts, the fish MT5-MMP and MT5-MMP-del both lack the signal peptide and a part of the prodomain. The fish MT5-MMP and MT5-MMP-del were different in that the latter did not have the stem/transmembrane/cytoplasmic domain. The two fish MMPs were expressed in the ovary, testis, brain, and intestine. In the ovary, MT5-MMP mRNA was expressed in the oocytes of small growing follicles. In contrast, MT5-MMP-del mRNA was found in the stromal interstitial cells. These results strongly suggest that a MT5-MMP/gelatinase A cascade may possibly operate in the process of spawning and/or other events associated with ovulated oocytes or fertilized eggs of the medaka fish.
A Kimura; M Shinohara; R Ohkura; T Takahashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1518     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-27     Completed Date:  2001-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  115-23     Citation Subset:  IM    
Division of Biological Sciences, Graduate School of Science, Hokkaido University, 060-0810, Sapporo, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB047650;  AB047651
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MeSH Terms
3' Untranslated Regions
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Complementary
Gene Expression
Matrix Metalloproteinases, Membrane-Associated
Metalloendopeptidases / genetics*
Molecular Sequence Data
Ovary / enzymology*
Polymerase Chain Reaction / methods
RNA, Messenger*
Sequence Homology, Amino Acid
Tissue Distribution
Reg. No./Substance:
0/3' Untranslated Regions; 0/DNA, Complementary; 0/RNA, Messenger; EC 3.4.24.-/MMP24 protein, human; EC 3.4.24.-/Matrix Metalloproteinases, Membrane-Associated; EC 3.4.24.-/Metalloendopeptidases; EC 3.4.24.-/Mmp24 protein, mouse

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