Document Detail


Expression and localization of prohormone convertase PC1 in the calcitonin-producing cells of the bullfrog ultimobranchial gland.
MedLine Citation:
PMID:  14566018     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We examined the expression and localization of the prohormone convertases, PC1 and PC2, in the ultimobranchial gland of the adult bullfrog using immunohistochemical (IHC) and in situ hybridization (ISH) techniques. In the ultimobranchial gland, PC1-immunoreactive cells were columnar, and were present in the follicular epithelium. When serial sections were immunostained with anti-calcitonin, anti-CGRP, anti-PC1, and anti-PC2 sera, PC1 was found only in the calcitonin/CGRP-producing cells. No PC2-immunopositive cells were detected. In the ISH, PC1 mRNA-positive cells were detected in the follicle cells in the ultimobranchial gland. No PC2 mRNA-positive cells were detected. RT-PCR revealed expression of the mRNAs of PC1 and the PC2 in the ultimobranchial gland. However, very little of the PC2 mRNA is probably translated because no PC2 protein was detected either by IHC staining or by Western blotting analysis. We conclude that the main prohormone convertase that is involved in the proteolytic cleavage of procalcitonin in the bullfrog is PC1.
Authors:
Yuichi Yaoi; Masakazu Suzuki; Hideaki Tomura; Shingo Kurabuchi; Yuichi Sasayama; Shigeyasu Tanaka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  51     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-10-20     Completed Date:  2003-12-23     Revised Date:  2014-06-10    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1459-66     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibody Specificity
Calcitonin / biosynthesis*
Immunohistochemistry / methods
Proprotein Convertase 1 / biosynthesis*,  genetics,  metabolism
Proprotein Convertase 2 / biosynthesis,  genetics,  metabolism
RNA, Messenger / metabolism
Rana catesbeiana
Reverse Transcriptase Polymerase Chain Reaction
Ultimobranchial Body / cytology,  enzymology,  metabolism*
Chemical
Reg. No./Substance:
0/RNA, Messenger; 9007-12-9/Calcitonin; EC 3.4.21.93/Proprotein Convertase 1; EC 3.4.21.94/Proprotein Convertase 2
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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