Document Detail


Expression and localization of the progesterone receptor in mouse and human reproductive organs.
MedLine Citation:
PMID:  17170211     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of gonadotropins on progesterone receptor (PR) expression and localization in the mouse oviduct, uterus, and ovary was examined. In the oviduct ciliated epithelial cells of adult mice and human revealed a unique PR localization to the lower half of the motile cilia whereas the nuclei were unstained or faintly stained. Pubertal female mice were further studied by confocal laser scanning microscopy and western blotting before and after injection with FSH and LH followed by human chorionic gonadotropin (hCG) injection after a 48-h period. PR immunolocalization to the oviduct cilia was greatly increased in pubertal mice upon hCG stimulation. In neighboring goblet cells, the PR staining was confined to the nuclei. Nuclear PR localization was evident in epithelial cells of the uterus as well as in a fraction of stromal and muscle cells. Staining intensity and number of stained cells was not affected by hormone stimulation. In the ovary, weak PR immunolocalization was observed in unprimed animals but increased significantly after hCG stimulation. In granulosa cells of preovulatory follicles PR was exclusively observed in mural cells, whereas cumulus cells remained negative. At all stages examined, primary granulosa cell cilia lacked PR staining. SDS-PAGE and western blotting analysis of tissues from oviduct, uterus, and ovary confirmed antibody specificity, and identified two bands corresponding to the PR isoforms PR-A and PR-B. Upon hCG stimulation, a new band cross-reacting with anti-PR emerged above the PR-A form in oviduct fractions, suggesting LH-induced phosphorylation of PR-A. We suggest that ciliary PR in the oviduct plays a role in progesterone signaling after ovulation, possibly via non-genomic events. These novel findings warrant further studies of oviduct and postovulatory signaling events and suggest a sensory role for oviduct cilia in the process of oocyte transport/fertilization.
Authors:
Stefan Cuoni Teilmann; Christian Alexandro Clement; Jørgen Thorup; Anne Grete Byskov; Søren Tvorup Christensen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of endocrinology     Volume:  191     ISSN:  0022-0795     ISO Abbreviation:  J. Endocrinol.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-12-15     Completed Date:  2007-02-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  England    
Other Details:
Languages:  eng     Pagination:  525-35     Citation Subset:  IM    
Affiliation:
Laboratory of Reproductive Biology, Juliane Marie Center, Rigshospitalet, Copenhagen, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Blotting, Western / methods
Cell Nucleus / chemistry
Chorionic Gonadotropin / pharmacology
Cytoplasm / chemistry
Electrophoresis, Polyacrylamide Gel
Epithelial Cells / chemistry
Fallopian Tubes / chemistry
Female
Gene Expression
Genitalia, Female / chemistry*
Granulosa Cells / chemistry
Humans
Immunohistochemistry / methods
Mice
Mice, Inbred C57BL
Microscopy, Confocal
Ovary / chemistry
Receptors, Progesterone / analysis*,  genetics
Uterus / chemistry
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Receptors, Progesterone

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