Document Detail


Expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in human preeclamptic placenta: possible implications in the process of trophoblast apoptosis.
MedLine Citation:
PMID:  15708124     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) was originally identified as a receptor for oxidatively modified low-density lipoprotein. It has been reported that oxidative stress and hyperlipidemia play important roles in the etiology of preeclampsia, and that placental oxidative stress may stimulate syncytiotrophoblast apoptosis in preeclampsia. In this study, we examined the expression of LOX-1 in the human placentas of normal pregnancies and in preeclampsia using immunohistochemistry and Western blot analysis, and proposed that LOX-1 has a role in trophoblast apoptosis. To analyze apoptotic activity, the expression of the specific caspase cleavage site within cytokeratin 18 was assessed immunohistochemically using the monoclonal antibody M30 CytoDeath. Both LOX-1 and M30 immunoreactivity occurred predominantly in syncytiotrophoblasts. A significantly higher number of LOX-1 and M30-positive cells were found in preeclamptic placentas than in normal placentas. The number of M30-positive cells correlated with the apoptotic index of trophoblasts determined by TdT-mediated dUTP nick-end labeling (TUNEL). Syncytiotrophoblasts showing apoptotic activity were immunopositive to LOX-1 by double immunohistochemical fluorescence. We suggest that the functional role of syncytiotrophoblasts in placental dysfunction results from the localization and upregulation of LOX-1 in the preeclamptic placenta, possible implications in upregulation of syncytiotrophoblast apoptotic activity in preeclampsia.
Authors:
H Lee; H Park; Y J Kim; H J Kim; Y M Ahn; B Park; J H Park; B Eun Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Placenta     Volume:  26     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:    2005 Feb-Mar
Date Detail:
Created Date:  2005-02-14     Completed Date:  2005-07-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  226-33     Citation Subset:  IM    
Copyright Information:
(c) Elsevier Ltd. All rights reserved.
Affiliation:
Department of Anatomy, Ewha Womans University Medical College, Republic of Korea. hylee38@ewha.ac.kr
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MeSH Terms
Descriptor/Qualifier:
Adult
Antibodies, Monoclonal
Apoptosis*
Blotting, Western
Caspases / metabolism
Cell Count
Female
Fluorescent Antibody Technique, Indirect
Humans
Immunoenzyme Techniques
In Situ Nick-End Labeling
Keratins / immunology,  metabolism
Pre-Eclampsia / metabolism*,  pathology
Pregnancy
Receptors, LDL / metabolism*
Receptors, Oxidized LDL
Scavenger Receptors, Class E
Trophoblasts / metabolism*,  pathology
Up-Regulation
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/OLR1 protein, human; 0/Receptors, LDL; 0/Receptors, Oxidized LDL; 0/Scavenger Receptors, Class E; 68238-35-7/Keratins; EC 3.4.22.-/Caspases

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