| Expression of laminin-2 by normal and neoplastic rat C cells during the development of medullary thyroid carcinoma. | |
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MedLine Citation:
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PMID: 10335943 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Medullary thyroid carcinoma (MTC) originates from C cells, which secrete calcitonin (CT), their specific marker. C cells are located in contact with the basement membrane (BM) of the thyroid follicles, which is partly made up of the laminin-2 isoform synthesized by thyrocytes. During oncogenesis, proliferation of the C cells, invading the centre of the follicles, leads to a break in their normal contact with the BM. As specific interactions of cells with BM components, especially laminins, are important for proliferation and differentiation, we investigated the relationships of normal and neoplastic C cells with laminin in the Wag/Rij rat model of human MTC. Immunocytochemical studies showed a progressive loss of the laminin layer underlying the hyperplastic C cell nodules around the large dedifferentiated tumours. The alpha2, beta1 and gamma1 chains of the laminin-2 isoform were synthesized and secreted by rat MTC 6-23 cell cultures and the tumours induced by subcutaneous injection of these cells. In situ hybridization combined with anti-CT immunocytochemistry showed a low expression of alpha2 mRNA on differentiated C cells and thyrocytes, but an overexpression on immunonegative spontaneous MTC and induced intrathyroid tumours. The high level of alpha2 gene expression, together with tumour dedifferentiation, suggests a relationship with malignancy. |
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Authors:
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F Lekmine; H Feracci; G Milhaud; F Treilhou-Lahille; N Jeanne |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Virchows Archiv : an international journal of pathology Volume: 434 ISSN: 0945-6317 ISO Abbreviation: Virchows Arch. Publication Date: 1999 Apr |
Date Detail:
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Created Date: 1999-06-03 Completed Date: 1999-06-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9423843 Medline TA: Virchows Arch Country: GERMANY |
Other Details:
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Languages: eng Pagination: 325-32 Citation Subset: IM |
Affiliation:
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Laboratoire d'Endocrinologie Cellulaire et Evolution, URA 1116 CNRS, Université Paris-Sud, Orsay, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Basement Membrane / metabolism, pathology Carcinoma, Medullary / metabolism*, pathology DNA Primers / chemistry Disease Models, Animal Female In Situ Hybridization Laminin / biosynthesis*, genetics Male Neoplasm Transplantation Neoplasms, Experimental / metabolism, pathology RNA, Messenger / biosynthesis Rats Rats, Inbred Strains Reverse Transcriptase Polymerase Chain Reaction Thyroid Gland / metabolism*, pathology Thyroid Neoplasms / metabolism*, pathology Tumor Cells, Cultured / metabolism, pathology |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Laminin; 0/RNA, Messenger; 0/laminin alpha 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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