| Expression and kinetic changes of alkaline phosphatase and its isoenzymes in experimental rat hepatoma. | |
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MedLine Citation:
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PMID: 11819310 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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AIM:To explore the expression and changes of hepatoma specific alkaline phospatase (ALP) in rats during canceration.METHODS:The ALPs and isoenzymes of rat livers and sera were investigated in SD hepatomas induced with 0.05% 2-fluoenylacetamide (2-FAA).RESULTS: By pathological examination and biochemical analysis. ALPs were overexpressed in rat livers during canceration and then were secreted into blood. Serum total ALP activities, liver ALP specific activities (U/g) including soluble and membrane-combined ALP activities of each group were all significantly higher (P < 0.01)than those of control group. The average ratios of soluble ALP to membrane-combined ALP were increased significantly after 6 weeks. ALP isoenzymes of rat sera and livers showed 5 bands on PAGE:ALP-I and ALP-II were specific for normal liver and rat hepatoma tissues, the ALP-II appeared in rat liver after 6 weeks and in sera after 8 weeks.CONCLUSION:ALP with carcino-embryonic protein was overexpressed in hepatoma tissues; the abnormal ALP-II of ALP isoenzymes in sera and liver of rats can be used as a tumor marker for early diagnosis of rat hepatoma. |
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Authors:
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Ming-Bing Xiao; Deng-Fu Yao; Hong Zhang; Hui Qiang; Jie-Fei Huang; Qun Wei; Feng Jiang |
Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 4 ISSN: - ISO Abbreviation: World J. Gastroenterol. Publication Date: 1998 Aug |
Date Detail:
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Created Date: 2002-Jan-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: - |
Other Details:
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Languages: ENG Pagination: 323-325 Citation Subset: - |
Affiliation:
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Department of Gastroenterology, Affiliated Hospital, Nantong Medical College,No.20 Xisi Road,Nantong 226001,Jiangsu Province,China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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