Document Detail


Expression of kallikrein-related peptidase 4 in dental and non-dental tissues.
MedLine Citation:
PMID:  22243250     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Kallikrein-related peptidase 4 (KLK4) is critical for proper dental enamel formation. Klk4 null mice, and humans with two defective KLK4 alleles have obvious enamel defects, with no other apparent phenotype. KLK4 mRNA or protein is reported to be present in tissues besides teeth, including prostate, ovary, kidney, liver, and salivary gland. In this study we used the Klk4 knockout/NLS-lacZ knockin mouse to assay Klk4 expression using β-galactosidase histochemistry. Incubations for 5 h were used to detect KLK4 expression with minimal endogenous background, while overnight incubations susceptible to false positives were used to look for trace KLK4 expression. Developing maxillary molars at postnatal days 5, 6, 7, 8, and 14, developing mandibular incisors at postnatal day 14, and selected non-dental tissues from adult wild-type and Klk4(lacZ/lacZ) mice were examined by X-gal histochemistry. After 5 h of incubation, X-gal staining was observed specifically in the nuclei of maturation-stage ameloblasts in molars and incisors from Klk4(lacZ/lacZ) mice and was detected weakly in the nuclei of salivary gland ducts and in patches of prostate epithelia. We conclude that KLK4 is predominantly a tooth-specific protease with low expression in submandibular salivary gland and prostate, and with no detectable expression in liver, kidney, testis, ovary, oviduct, epididymis, and vas deferens.
Authors:
James P Simmer; Amelia S Richardson; Charles E Smith; Yuanyuan Hu; Jan C-C Hu
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  European journal of oral sciences     Volume:  119 Suppl 1     ISSN:  1600-0722     ISO Abbreviation:  Eur. J. Oral Sci.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2012-01-16     Completed Date:  2012-06-18     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  9504563     Medline TA:  Eur J Oral Sci     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  226-33     Citation Subset:  D; IM    
Copyright Information:
© 2011 Eur J Oral Sci.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Ameloblasts / enzymology*
Amelogenesis
Animals
Dental Enamel / enzymology*
Female
Gene Knock-In Techniques
Gene Knockout Techniques
Kallikreins / analysis,  biosynthesis*,  genetics
Male
Mice
Mice, Knockout
Organ Specificity
Prostate / enzymology*
Submandibular Gland / enzymology*
beta-Galactosidase / genetics
Grant Support
ID/Acronym/Agency:
DE019775/DE/NIDCR NIH HHS; R01 DE019775/DE/NIDCR NIH HHS; R01 DE019775-04/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
EC 3.2.1.23/beta-Galactosidase; EC 3.4.21.-/Kallikreins; EC 3.4.21.-/kallikrein 4
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Kallikrein-related peptidase 4, matrix metalloproteinase 20, and the maturation of murine and porcin...
Next Document:  Characterization of kallikrein-related peptidase 4 glycosylations.