Document Detail


Expression of the jun family of genes in human ovarian cancer and normal ovarian surface epithelium.
MedLine Citation:
PMID:  8649827     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The jun genes (c-jun, jun-B and jun-D) play a role in critical cell functions such as proliferation, differentiation and apoptosis. We documented jun expression at the mRNA and protein level in human ovarian cancer tissues (n=28), surface epithelial cells of normal ovaries (n=14) and ovarian cancer cell lines (n=6). Almost all of ovarian tumors as well as normal ovaries concomitantly express c-jun, jun-B, and jun-D mRNA. Immunohistochemistry was less sensitive and revealed nuclear c-Jun and Jun-B proteins in the malignant epithelial cells of respectively 38% and 11% of ovarian tumors and in the surface epithelium of a normal premenopausal ovary. In cultured ovarian cancer cells, c-jun and jun-B expression is inducible by serum and TPA and is therefore not constitutive. The c-jun and jun-B proteins therefore play a role both in differentiation of the normal ovarian surface epithelium, as well as in the proliferation of epithelial ovarian cancer cells. High jun-B expression relates to a more malignant phenotype both in vitro and in vivo. The jun-D gene is suppressed in ovarian cancer cells as compared to normal ovarian surface epithelial cells in situ and in vitro. Downregulation of jun-D might therefore be part of the malignant ovarian epithelial cell phenotype.
Authors:
B Neyns; Katesuwanasing; J Vermeij; C Bourgain; B Vandamme; K Amfo; W Lissens; P DeSutter; E Hooghe-Peters; J DeGrève
Related Documents :
1877597 - Tubal sterilization, hysterectomy, and the subsequent occurrence of epithelial ovarian ...
2305307 - Survival after malignant pericardial effusion and cardiac tamponade in advanced ovarian...
25429527 - Diet, exercise, obesity, smoking and alcohol consumption in cancer survivors and the ge...
9815777 - Overexpression of epithelial macrophage colony-stimulating factor (csf-1) and csf-1 rec...
18974247 - Myeloid-derived suppressor cells in cancer cachexia syndrome: a new explanation for an ...
1010857 - Cancerline: a new nlm/nci data base.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncogene     Volume:  12     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-07-25     Completed Date:  1996-07-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1247-57     Citation Subset:  IM    
Affiliation:
Department of Medical Onocolgy and Haematology, Vrije Universiteit, Brussel, Belgium.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
3T3 Cells / metabolism,  physiology
Animals
Blotting, Northern
Epithelium / metabolism,  physiology
Female
Gene Expression / drug effects
Gene Expression Regulation, Neoplastic / drug effects
Genes, jun*
Humans
Immunohistochemistry
Mice
Ovarian Neoplasms / genetics,  metabolism*
Ovary / metabolism*,  physiology
Phenotype
Proto-Oncogene Proteins c-jun / biosynthesis*
RNA, Messenger / genetics,  metabolism
Reference Values
Tetradecanoylphorbol Acetate / pharmacology
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 16561-29-8/Tetradecanoylphorbol Acetate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  p16 inhibition of transformed and primary squamous epithelial cells.
Next Document:  Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning.