Document Detail

Expression of the jun-B gene during induction of monocytic differentiation.
MedLine Citation:
PMID:  2007095     Owner:  NLM     Status:  MEDLINE    
The AP-1 protein complex binds to specific DNA sequences that regulate transcription of genes responsive to certain growth factors and phorbol esters. This complex is composed of products of the jun and fos gene families. The present studies have examined the effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) on the regulation of jun-B gene expression during induction of monocytic differentiation. Low levels of jun-B transcripts were present in uninduced HL-60 promyelocytic leukemia cells. In contrast, treatment with TPA was associated with rapid increases in jun-B mRNA levels that were maximal at 3 h and remained elevated at 48 h. The induction of jun-B expression by TPA in these cells preceded that of the c-jun and c-fos genes. Similar increases in jun-B transcripts were detectable in TPA-treated THP-1 and U-937 myeloid leukemia cells, although expression of this gene was transient in the more differentiated THP-1 cells. Run-on assays demonstrated low levels of jun-B gene activation in untreated HL-60 cells, whereas TPA treatment was associated with a 6-fold increase in the transcription rate of this gene. This induction of jun-B expression occurred in the absence of de novo protein synthesis. In contrast, inhibition of protein synthesis was associated with superinduction of TPA-induced jun-B mRNA levels and an increase in stability of this transcript. These findings suggest that jun-B gene expression is regulated at both the transcriptional and posttranscriptional levels during induction of monocytic differentiation.(ABSTRACT TRUNCATED AT 250 WORDS)
R Datta; M L Sherman; R M Stone; D Kufe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research     Volume:  2     ISSN:  1044-9523     ISO Abbreviation:  Cell Growth Differ.     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-05-01     Completed Date:  1991-05-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9100024     Medline TA:  Cell Growth Differ     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  43-9     Citation Subset:  IM    
Laboratory of Clinical Pharmacology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.
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MeSH Terms
Calcitriol / pharmacology
Cell Differentiation / drug effects,  genetics
DNA-Binding Proteins / biosynthesis*
Gene Expression / physiology
Lactones / pharmacology
Leukemia, Myeloid / genetics
Monocytes / cytology,  drug effects,  metabolism*
Proto-Oncogene Proteins c-jun
Proto-Oncogenes / genetics*
Tetradecanoylphorbol Acetate / pharmacology
Transcription, Genetic / drug effects
Grant Support
Reg. No./Substance:
0/Bryostatins; 0/DNA-Binding Proteins; 0/Lactones; 0/Macrolides; 0/Proto-Oncogene Proteins c-jun; 16561-29-8/Tetradecanoylphorbol Acetate; 32222-06-3/Calcitriol; 83314-01-6/bryostatin 1

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