| Expression of isopentenyl transferase gene (ipt) in leaf and stem delayed leaf senescence without affecting root growth. | |
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MedLine Citation:
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PMID: 19820948 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A cytokinin biosynthetic gene encoding isopentenyl transferase (ipt) was cloned with its native promoter from Agrobacterium tumefaciens and introduced into tobacco plants. Indolebutyric acid was applied in rooting medium and morphologically normal transgenic tobacco plants were regenerated. Genetic analysis of self-fertilized progeny showed that a single copy of intact ipt gene had been integrated, and T(2) progeny had become homozygous for the transgene. Stable inheritance of the intact ipt gene in T(2) progeny was verified by Southern hybridization. Northern blot hybridization revealed that the expression of this ipt gene was confined in leaves and stems but undetectable in roots of the transgenic plants. Endogenous cytokinin levels in the leaves and stems of the transgenic tobaccos were two to threefold higher than that of control, but in roots, both the transgenic and control tobaccos had similar cytokinin levels. The elevated cytokinin levels in the transgenic tobacco leaves resulted in delayed leaf senescence in terms of chlorophyll content without affecting the net photosynthetic rate. The root growth and morphology of the plant were not affected in the transgenic tobacco. |
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Authors:
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Qing-Hu Ma; Yun-Chao Liu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-10 |
Journal Detail:
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Title: Plant cell reports Volume: 28 ISSN: 1432-203X ISO Abbreviation: Plant Cell Rep. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2010-01-29 Completed Date: 2010-04-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9880970 Medline TA: Plant Cell Rep Country: Germany |
Other Details:
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Languages: eng Pagination: 1759-65 Citation Subset: IM |
Affiliation:
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Institute of Botany, Chinese Academy of Sciences, Beijing, China. mqh@ibcas.ac.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkyl and Aryl Transferases
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genetics* Cytokinins / biosynthesis Gene Expression Regulation, Enzymologic* Gene Expression Regulation, Plant Phenotype Plant Leaves / enzymology, genetics, growth & development Plant Roots / enzymology*, genetics, growth & development Plant Stems / enzymology*, genetics, growth & development Plants, Genetically Modified Tobacco / enzymology*, genetics, growth & development |
| Chemical | |
Reg. No./Substance:
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0/Cytokinins; EC 2.5.-/Alkyl and Aryl Transferases; EC 2.5.1.27/adenylate isopentenyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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