Document Detail

Expression of interleukin-6 in osteoarthritic chondrocytes and effects of fluid-induced shear on this expression in normal human chondrocytes in vitro.
MedLine Citation:
PMID:  8618168     Owner:  NLM     Status:  MEDLINE    
This study tested the effect of fluid-induced shear on interleukin-6 expression in normal human articular chondrocytes in vitro. As determined by Northern blot analysis, interleukin-6 mRNA expression occurs in chondrocytes from osteoarthritic cartilage but not in normal chondrocytes. Applying fluid-induced shear stress to primary high density cultures of chondrocytes increased interleukin-6 mRNA signal 4-fold at 1 hour and 10 to 15-fold at 48 hours compared with unsheared control cultures. At 48 hours, fluid-induced shear stress increased interleukin-6 protein levels in the culture medium 9 to 10-fold compared with unsheared controls. mRNA signals for interleukin-1alpha, interleukin-1beta, and tumor necrosis factor-alpha in RNA from sheared or control chondrocytes were not detected by Northern blotting. Transforming growth factor-beta mRNA signal was detectable but was not affected by shear. In contrast, human lung fibroblasts (WI-38) responded to fluid-induced shear with increased signal for transforming growth factor-beta, but not interleukin-6, mRNA. Both cell types did respond to interleukin-1alpha with increased interleukin-6 mRNA signal. These data demonstrated that distortional forces, such as fluid-induced shear stress, alter interleukin-6 levels in normal chondrocytes in vitro and suggest that increased interleukin-6 expression in osteoarthritic cartilage may result, in part, from alterations in the mechanical loading of the tissue.
M Mohtai; M K Gupta; B Donlon; B Ellison; J Cooke; G Gibbons; D J Schurman; R L Smith
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of orthopaedic research : official publication of the Orthopaedic Research Society     Volume:  14     ISSN:  0736-0266     ISO Abbreviation:  J. Orthop. Res.     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1996-06-12     Completed Date:  1996-06-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8404726     Medline TA:  J Orthop Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  67-73     Citation Subset:  IM    
Department of Functional Restoration, Veterans Affairs Medical Center, Palo Alto, CA, USA.
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MeSH Terms
Blotting, Northern
Cartilage, Articular / cytology,  metabolism*,  pathology
Cells, Cultured
Child, Preschool
Fibroblasts / metabolism
Interleukin-1 / metabolism
Interleukin-6 / genetics,  metabolism*
Lung / cytology
Middle Aged
Osteoarthritis / metabolism*,  pathology
RNA, Messenger / metabolism
Reference Values
Stress, Mechanical
Time Factors
Transforming Growth Factor beta / metabolism
Tumor Necrosis Factor-alpha / metabolism
Reg. No./Substance:
0/Interleukin-1; 0/Interleukin-6; 0/RNA, Messenger; 0/Transforming Growth Factor beta; 0/Tumor Necrosis Factor-alpha

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