| Expression of interleukin-6 in osteoarthritic chondrocytes and effects of fluid-induced shear on this expression in normal human chondrocytes in vitro. | |
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MedLine Citation:
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PMID: 8618168 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study tested the effect of fluid-induced shear on interleukin-6 expression in normal human articular chondrocytes in vitro. As determined by Northern blot analysis, interleukin-6 mRNA expression occurs in chondrocytes from osteoarthritic cartilage but not in normal chondrocytes. Applying fluid-induced shear stress to primary high density cultures of chondrocytes increased interleukin-6 mRNA signal 4-fold at 1 hour and 10 to 15-fold at 48 hours compared with unsheared control cultures. At 48 hours, fluid-induced shear stress increased interleukin-6 protein levels in the culture medium 9 to 10-fold compared with unsheared controls. mRNA signals for interleukin-1alpha, interleukin-1beta, and tumor necrosis factor-alpha in RNA from sheared or control chondrocytes were not detected by Northern blotting. Transforming growth factor-beta mRNA signal was detectable but was not affected by shear. In contrast, human lung fibroblasts (WI-38) responded to fluid-induced shear with increased signal for transforming growth factor-beta, but not interleukin-6, mRNA. Both cell types did respond to interleukin-1alpha with increased interleukin-6 mRNA signal. These data demonstrated that distortional forces, such as fluid-induced shear stress, alter interleukin-6 levels in normal chondrocytes in vitro and suggest that increased interleukin-6 expression in osteoarthritic cartilage may result, in part, from alterations in the mechanical loading of the tissue. |
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Authors:
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M Mohtai; M K Gupta; B Donlon; B Ellison; J Cooke; G Gibbons; D J Schurman; R L Smith |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of orthopaedic research : official publication of the Orthopaedic Research Society Volume: 14 ISSN: 0736-0266 ISO Abbreviation: J. Orthop. Res. Publication Date: 1996 Jan |
Date Detail:
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Created Date: 1996-06-12 Completed Date: 1996-06-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8404726 Medline TA: J Orthop Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 67-73 Citation Subset: IM |
Affiliation:
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Department of Functional Restoration, Veterans Affairs Medical Center, Palo Alto, CA, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Blotting, Northern Cartilage, Articular / cytology, metabolism*, pathology Cells, Cultured Child, Preschool Female Fibroblasts / metabolism Humans Interleukin-1 / metabolism Interleukin-6 / genetics, metabolism* Lung / cytology Male Middle Aged Osteoarthritis / metabolism*, pathology RNA, Messenger / metabolism Reference Values Stress, Mechanical Time Factors Transforming Growth Factor beta / metabolism Tumor Necrosis Factor-alpha / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-1; 0/Interleukin-6; 0/RNA, Messenger; 0/Transforming Growth Factor beta; 0/Tumor Necrosis Factor-alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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