Document Detail


Expression of interferon regulatory factor 1, 3, and 7 in primary Sjögren syndrome.
MedLine Citation:
PMID:  19426920     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The aim was to investigate the level of interferon regulatory factor (IRF) 1, 3, and 7 in peripheral blood cells from patients with primary Sjogren syndrome (pSS) and to determine whether and where IRF1 exists in the parotid glands of pSS. METHODS: Peripheral blood cells and parotid gland biopsy specimens from patients with pSS were studied. The IRF1, IRF3, and IRF7 gene mRNA levels in peripheral blood cells were calculated by using real-time PCR. The IRF1-positive cells in the parotid glands with pSS were observed by using immunohistochemistry and immunofluorescence. Statistical analysis was performed by Student t test. RESULTS: Compared with 24 control samples, the IRF1 mRNA levels in peripheral blood cells of 37 cases with pSS were up-regulated (P < .05), but the IRF3 and IRF7 mRNA levels of pSS were not up-regulated (P > .05). Relative quantitative levels of IRF1 mRNA were 2.17-fold higher in pSS patients than control subjects. The IRF1-positive cells of the pSS group were localized in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands. In all control subjects, the IRF1-positive cells were localized only to the ductal epithelial cells of parotid glands as determined by immunohistochemical staining or immunofluorescence. The scores of IRF1-positive cells of pSS were significantly higher than that of control samples (P < .05). CONCLUSION: These findings indicate that IRF1 mRNA levels are up-regulated in the peripheral blood cells of pSS patients. Also, IRF1-positive cells exist in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands of individuals affected by pSS, but are limited to the ductal epithelial cells of healthy control subjects.
Authors:
Lingyan Zheng; Chuangqi Yu; Zhiyuan Zhang; Chi Yang; Xieyi Cai
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics     Volume:  107     ISSN:  1528-395X     ISO Abbreviation:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-11     Completed Date:  2009-08-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508562     Medline TA:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  661-8     Citation Subset:  D; IM    
Affiliation:
Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, School of Medicine, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University, Shanghai, China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Case-Control Studies
Epithelial Cells / metabolism
Female
Fluorescent Antibody Technique
Gene Expression
Humans
Interferon Regulatory Factor-1 / biosynthesis,  genetics
Interferon Regulatory Factor-3 / biosynthesis,  blood,  genetics
Interferon Regulatory Factor-7 / biosynthesis,  blood,  genetics
Interferon Regulatory Factors / biosynthesis*,  blood,  genetics
Lymphocytes / metabolism
Male
Middle Aged
Parotid Gland / metabolism
RNA, Messenger / blood
Reverse Transcriptase Polymerase Chain Reaction
Sjogren's Syndrome / blood,  genetics,  immunology*,  metabolism*
Up-Regulation
Young Adult
Chemical
Reg. No./Substance:
0/IRF1 protein, human; 0/IRF3 protein, human; 0/IRF7 protein, human; 0/Interferon Regulatory Factor-1; 0/Interferon Regulatory Factor-3; 0/Interferon Regulatory Factor-7; 0/Interferon Regulatory Factors; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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