| Expression of interferon gamma and proinflammatory cytokines in the cecal mucosa of rats experimentally infected with Blastocystis sp. strain RN94-9. | |
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MedLine Citation:
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PMID: 19255785 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Blastocystis hominis is a zoonotic intestinal protozoan parasite whose pathogenic potential is still controversial. The aim of the present study was to clarify the pathogenicity of Blastocystis parasites in rats. Oral inoculation with 1 x 10(5) cysts of Blastocystis sp. strain RN94-9 in rats resulted in chronic infection in the cecum at least until 4 weeks after infection. Histological examination revealed neither mucosal sloughing nor inflammatory cell infiltration but showed a slight but significant increase in goblet cell numbers in the cecal mucosa 1-3 weeks post-infection. Differential staining of acidic and neutral mucins by the alcian blue-periodic acid-Schiff method showed that the predominantly increased cells were neutral mucin(+) but not acidic mucin(+) goblet cells. Reverse transcription real-time polymerase chain reaction studies demonstrated significant upregulation of the expression of interferon-gamma, interleukin (IL)-12, and tumor necrosis factor alpha, but not IL-6 or granulocyte-macrophage colony-stimulating factor, in the cecal mucosa at 2 and/or 3 weeks post-infection. The induction of local host responses, including mild goblet cell hyperplasia, and significant upregulation of type-1 and proinflammatory cytokines, suggest that Blastocystis sp. strain RN94-9 is a weakly pathogenic organism that could elicit proinflammatory as well as protective responses in local tissues. |
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Authors:
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Aya Iguchi; Hisao Yoshikawa; Minoru Yamada; Isao Kimata; Naoki Arizono |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-03 |
Journal Detail:
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Title: Parasitology research Volume: 105 ISSN: 1432-1955 ISO Abbreviation: Parasitol. Res. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-05-22 Completed Date: 2009-07-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8703571 Medline TA: Parasitol Res Country: Germany |
Other Details:
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Languages: eng Pagination: 135-40 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Nara Women's University, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocystis / immunology*, pathogenicity Blastocystis Infections / immunology*, pathology Cecum / immunology*, pathology Cytokines / biosynthesis* Gene Expression Profiling Goblet Cells / pathology Intestinal Mucosa / immunology*, pathology Male Mucins / analysis Rats |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Mucins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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