Document Detail

Expression of intercellular adhesion molecule-1 (ICAM-1) on cultured human endometrial stromal cells and its role in the interaction with natural killers.
MedLine Citation:
PMID:  7880394     Owner:  NLM     Status:  MEDLINE    
PROBLEM: Recent evidence emphasizes the role of natural killer cells (NKs) as potential effectors of peritoneal immune surveillance directed against the outgrowth of endometrial cells, refluxed with menstrual debris, in ectopic sites. This NK-mediated cytotoxicity toward autologous endometrial antigens seems to be significantly decreased in endometriosis patients. METHOD: We set up experiments to clarify which molecules are involved in NK-endometrial cell interaction. In particular, we evaluated the surface expression and functional activity of intercellular adhesion molecule-1 (ICAM-1), a cell surface glycoprotein that has been identified as one of the ligands for lymphocyte function-associated antigen-1 (LFA-1), present on almost all leucocyte cell types. Immunofluorescence flow cytometry was used to assess ICAM-1 expression on resting and IL 1 beta-activated endometrial stromal cells in culture. Dermal fibroblasts were used as control cells. Cytotoxicity and binding assays by 51Cr release in presence and absence of a specific monoclonal antibody (mAb) against ICAM-1 were then performed in order to determine the effect of this molecule on NK-mediated cytotoxic and binding activity toward endometrial stromal cells. RESULTS: The results of this study indicated that ICAM-1 expression on endometrial stromal cells seems to be constitutively higher than on dermal fibroblasts and can be up-regulated upon exposure to IL 1 beta. Furthermore, a mAb against ICAM-1 strongly inhibits the binding but not the cytotoxicity of NKs toward endometrial cells. No difference in the expression of this molecule was observed throughout the cycle. CONCLUSIONS: The presence of ICAM-1 on human endometrium might relate to the action of the immunocompetent cells in human specific reproductive events.
P Viganó; R Pardi; B Magri; M Busacca; A M Di Blasio; M Vignali
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of reproductive immunology (New York, N.Y. : 1989)     Volume:  32     ISSN:  1046-7408     ISO Abbreviation:  Am. J. Reprod. Immunol.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1995-04-13     Completed Date:  1995-04-13     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8912860     Medline TA:  Am J Reprod Immunol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  139-45     Citation Subset:  IM    
II Dept. of Obstetrics and Gynecology, Clinica L. Mangiagalli, University of Milano, Italy.
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MeSH Terms
Cell Adhesion / immunology*
Cells, Cultured
Cytotoxicity Tests, Immunologic
Endometrium / cytology,  immunology*
Flow Cytometry
Intercellular Adhesion Molecule-1 / biosynthesis*,  immunology
Killer Cells, Natural / immunology*
Lymphocyte Function-Associated Antigen-1 / immunology
Stromal Cells / immunology*
Reg. No./Substance:
0/Lymphocyte Function-Associated Antigen-1; 126547-89-5/Intercellular Adhesion Molecule-1

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