| Expression of intercellular adhesion molecule-1 in U937 foam cells and inhibitory effect of imperatorin. | |
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MedLine Citation:
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PMID: 11931707 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To investigate the expression level of intercellular adhesion molecule-1 (ICAM-1) in a macrophage-derived foam cell model from human U937 cell line and the inhibitory effect of imperatorin (IMP) on the ICAM-1. METHODS: U937 cells were incubated with oxidized low density lipoprotein (ox-LDL) 80 mg/L for 48 h and a macrophage-derived foam cell model was established. The medium was pretreated with different concentrations of IMP (0, 25, 50, 100 micromol/L). ICAM-1 protein expression in cells was measured with Western blotting; ICAM-1 mRNA level in cells was measured by Northern blotting. RESULTS: After incubated with ox-LDL, ICAM-1 expression level increased greatly. The increase in ICAM-1 protein level and mRNA level was estimated to be about 15-fold and 10-fold. When the cells were pretreated with imperatorin (50, 100 micromol/L), the increase of ICAM-1 in foam cells were remarkably inhibited. Especially when pretreated with IMP 100 micromol/L, the ICAM-1 protein level decreased by 79 % and the mRNA level decreased by 74 % each compared to the level of foam cells. CONCLUSION: After incubated with ox-LDL in vitro, the U937 foam cells showed an enhanced ICAM-1 expression compared with normal U937 cells. IMP could inhibit the expression of ICAM-1 in U937 foam cells. |
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Authors:
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Peng-Yuan Yang; Yao-Cheng Rui; Kai Li; Xing-Hua Huang; Jian-Ming Jiang; Long Yu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta pharmacologica Sinica Volume: 23 ISSN: 1671-4083 ISO Abbreviation: Acta Pharmacol. Sin. Publication Date: 2002 Apr |
Date Detail:
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Created Date: 2002-04-04 Completed Date: 2003-09-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100956087 Medline TA: Acta Pharmacol Sin Country: China |
Other Details:
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Languages: eng Pagination: 327-30 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, School of Pharmacy; Department of Thoracic Cardiac Surgery, Changhai Hospital, The Second Medical Military University, Shanghai 200433, China. Ruiyc@smmu.edu.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Angelica
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chemistry Depression, Chemical Foam Cells / drug effects*, metabolism Gene Expression / drug effects Humans Intercellular Adhesion Molecule-1 / biosynthesis*, genetics Lipoproteins, LDL / pharmacology Plant Roots / chemistry Psoralens / pharmacology* U937 Cells |
| Chemical | |
Reg. No./Substance:
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0/Lipoproteins, LDL; 0/Psoralens; 0/oxidized low density lipoprotein; 126547-89-5/Intercellular Adhesion Molecule-1; 482-44-0/imperatorin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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