Document Detail

Expression of intercellular adhesion molecule-1 in U937 foam cells and inhibitory effect of imperatorin.
MedLine Citation:
PMID:  11931707     Owner:  NLM     Status:  MEDLINE    
AIM: To investigate the expression level of intercellular adhesion molecule-1 (ICAM-1) in a macrophage-derived foam cell model from human U937 cell line and the inhibitory effect of imperatorin (IMP) on the ICAM-1. METHODS: U937 cells were incubated with oxidized low density lipoprotein (ox-LDL) 80 mg/L for 48 h and a macrophage-derived foam cell model was established. The medium was pretreated with different concentrations of IMP (0, 25, 50, 100 micromol/L). ICAM-1 protein expression in cells was measured with Western blotting; ICAM-1 mRNA level in cells was measured by Northern blotting. RESULTS: After incubated with ox-LDL, ICAM-1 expression level increased greatly. The increase in ICAM-1 protein level and mRNA level was estimated to be about 15-fold and 10-fold. When the cells were pretreated with imperatorin (50, 100 micromol/L), the increase of ICAM-1 in foam cells were remarkably inhibited. Especially when pretreated with IMP 100 micromol/L, the ICAM-1 protein level decreased by 79 % and the mRNA level decreased by 74 % each compared to the level of foam cells. CONCLUSION: After incubated with ox-LDL in vitro, the U937 foam cells showed an enhanced ICAM-1 expression compared with normal U937 cells. IMP could inhibit the expression of ICAM-1 in U937 foam cells.
Peng-Yuan Yang; Yao-Cheng Rui; Kai Li; Xing-Hua Huang; Jian-Ming Jiang; Long Yu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  23     ISSN:  1671-4083     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-04-04     Completed Date:  2003-09-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  327-30     Citation Subset:  IM    
Department of Pharmacology, School of Pharmacy; Department of Thoracic Cardiac Surgery, Changhai Hospital, The Second Medical Military University, Shanghai 200433, China.
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MeSH Terms
Angelica / chemistry
Depression, Chemical
Foam Cells / drug effects*,  metabolism
Gene Expression / drug effects
Intercellular Adhesion Molecule-1 / biosynthesis*,  genetics
Lipoproteins, LDL / pharmacology
Plant Roots / chemistry
Psoralens / pharmacology*
U937 Cells
Reg. No./Substance:
0/Lipoproteins, LDL; 0/Psoralens; 0/oxidized low density lipoprotein; 126547-89-5/Intercellular Adhesion Molecule-1; 482-44-0/imperatorin

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