Document Detail

Expression of inter-alpha-trypsin inhibitor heavy chains in endometrium of cyclic and pregnant gilts.
MedLine Citation:
PMID:  14611635     Owner:  NLM     Status:  MEDLINE    
Attachment of the placenta to the uterus in pigs involves extracellular interaction between the expanding trophoblastic membrane and the thick glycocalyx present on the uterine epithelial microvilli. Formation of complexes between members of inter-alpha-trypsin inhibitor family may function in the maintenance of the extracellular matrix. This study investigated the change in the inter-alpha-trypsin inhibitor heavy chains (ITIH1, ITIH2, ITIH3 and ITIH4) during the oestrous cycle and early pregnancy in pigs. Gene expression of ITIH1, ITIH2, ITIH3 and ITIH4 was detected in the endometrium of cyclic and pregnant gilts; however, gene expression of ITIH was not altered throughout the oestrous cycle or early pregnancy. Western blot analysis with an ITIH antiserum identified the possible linkage forms of ITIH with the serine protease inhibitor, bikunin. Pregnancy altered the release of the various inter-alpha-inhibitor forms from the endometrium during the period of trophoblastic attachment. The results from this study indicate that the inter-alpha-trypsin inhibitor family plays an important role in maintenance of the uterine surface glycocalyx during placental attachment in pigs.
R D Geisert; M D Ashworth; J R Malayer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  126     ISSN:  1470-1626     ISO Abbreviation:  Reproduction     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-11-12     Completed Date:  2004-02-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  621-7     Citation Subset:  IM    
Department of Animal Science, Oklahoma Agriculture Experiment Station, Oklahoma State University, Stillwater, OK 74078, USA.
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MeSH Terms
Acute-Phase Proteins / genetics
Alpha-Globulins / analysis,  genetics*
Endometrium / chemistry,  metabolism*
Estrus / metabolism
Gene Expression
Placentation / physiology
Pregnancy, Animal / metabolism*
Protein Precursors / genetics
RNA, Messenger / analysis*
Reverse Transcriptase Polymerase Chain Reaction
Swine / metabolism*
Trypsin Inhibitors / genetics
Reg. No./Substance:
0/Acute-Phase Proteins; 0/Alpha-Globulins; 0/MAP protein, Sus scrofa; 0/Protein Precursors; 0/RNA, Messenger; 0/Trypsin Inhibitors; 39346-44-6/inter-alpha-inhibitor

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