| Expression of integrin receptors and ICAM-1 on keratinocytes in vivo and in an in vitro reconstructed epidermis: effect of sodium dodecyl sulphate. | |
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MedLine Citation:
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PMID: 7598528 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several integrin heterodimers such as alpha 2 beta 1, alpha 6 beta 4 and alpha v beta 5 are expressed on basal keratinocytes of the epidermis. Because overexpression of these integrins as well as induction of the intercellular adhesion molecule-1 (ICAM-1) have been found in inflammatory dermatoses, we sought to determine whether these modulations could be used as markers of skin irritation. In normal epidermis, topical application of 1% sodium dodecyl sulphate (SDS) for 24 h caused an upregulation of alpha 3, beta 1, alpha 6, beta 4, alpha v, beta 5 and to a lesser extent alpha 2 integrin chains as well as an induction of ICAM-1. To investigate whether these parameters could also be used for evaluation of skin irritancy in vitro, SDS was applied for 24 h to reconstructed epidermis on de-epidermized dermis (RE-DED). In RE-DED, integrin overexpression and aberrant alpha 5 expression was seen under normal in vitro culture conditions and topical application of SDS caused only marginal additional upregulation. We could not detect any ICAM-1 reactivity on either normal or irritated RE-DED. Our results demonstrate that the modulation of integrin and ICAM-1 expression can be used as markers of irritation of the epidermis in vivo, but not in vitro. |
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Authors:
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P von den Driesch; M Fartasch; A Hüner; M Ponec |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of dermatological research Volume: 287 ISSN: 0340-3696 ISO Abbreviation: Arch. Dermatol. Res. Publication Date: 1995 |
Date Detail:
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Created Date: 1995-08-03 Completed Date: 1995-08-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8000462 Medline TA: Arch Dermatol Res Country: GERMANY |
Other Details:
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Languages: eng Pagination: 249-53 Citation Subset: IM |
Affiliation:
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Department of Dermatology, University of Erlangen-Nürnberg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Markers Dermatitis, Contact / etiology Drug Evaluation, Preclinical / methods Epidermis / drug effects*, metabolism*, pathology Humans Immunohistochemistry Integrins / metabolism* Intercellular Adhesion Molecule-1 / metabolism* Irritants / toxicity Keratinocytes / drug effects*, metabolism*, pathology Sodium Dodecyl Sulfate / toxicity* Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Integrins; 0/Irritants; 126547-89-5/Intercellular Adhesion Molecule-1; 151-21-3/Sodium Dodecyl Sulfate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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