| Expression of insect (Microdera puntipennis dzungarica) antifreeze protein MpAFP149 confers the cold tolerance to transgenic tobacco. | |
| | |
MedLine Citation:
|
PMID: 18528694 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To elucidate the function of antifreeze protein from Microdera puntipennis dzhungarica for freezing stress tolerance in plant, the construct of MpAFP149 gene with the signal peptide sequence responsible for secreting the native MpAFP149 into the apoplast space under control of a cauliflower mosaic virus 35S promoter was introduced into tobacco by Agrobacterium tumefaciens-mediated transformation. The observation of immunogold localization by TEM (transmission electron microscope) showed that the heterologous MpAFP149 protein was mainly distributed on the cell wall in apoplast of the transgenic tobacco plant. T1 generation transgenic tobacco plants displayed a more frost resistant phenotype and kept the lower ion leakage ratio and MDA (malondialdehyde) content in the leaves compared with wild-type ones at -1 degrees C for 3 days. The results showed that MpAFP149 provided protection and conferred cold tolerance to transgenic tobacco plant during freezing stress. |
| | |
Authors:
|
Yan Wang; Liming Qiu; Chunying Dai; Jing Wang; Jianmin Luo; Fuchun Zhang; Ji Ma |
Related Documents
:
|
15251044 - Two-fold differences are the detection limit for determining transgene copy numbers in ... 12029464 - Overexpression of the arabidopsis thaliana mine1 bacterial division inhibitor homologue... 12228344 - Selection and characterization of [alpha]-methyltryptophan-resistant lines of lemna gib... 10398704 - Reduced activity of geranylgeranyl reductase leads to loss of chlorophyll and tocophero... 11936644 - Real time control of a combined uasb-activated sludge wastewater treatment configuration. 23206124 - Sumo conjugating enzyme with active site mutation acts as dominant negative inhibitor o... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-06-05 |
Journal Detail:
|
Title: Plant cell reports Volume: 27 ISSN: 0721-7714 ISO Abbreviation: Plant Cell Rep. Publication Date: 2008 Aug |
Date Detail:
|
Created Date: 2008-07-03 Completed Date: 2008-11-26 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9880970 Medline TA: Plant Cell Rep Country: Germany |
Other Details:
|
Languages: eng Pagination: 1349-58 Citation Subset: IM |
Affiliation:
|
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 830046, Urumqi, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adaptation, Physiological
/
genetics,
physiology Animals Antifreeze Proteins / genetics*, physiology Beetles / genetics, metabolism Caulimovirus / genetics Freezing* Gene Expression Regulation, Plant Insect Proteins / genetics, physiology Microscopy, Electron, Transmission Plants, Genetically Modified / genetics*, physiology, ultrastructure Promoter Regions, Genetic / genetics Tobacco / genetics*, physiology, ultrastructure |
| Chemical | |
Reg. No./Substance:
|
0/Antifreeze Proteins; 0/Insect Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Peripheral neuropathies of the forearm and hand in rheumatoid arthritis: diagnosis and options for t...
Next Document: Pulmonary inflammatory myofibroblastic tumor after Hodgkin's lymphoma and application of PET imaging...