| Expression of the Na+/myo-inositol cotransporter in the juxtaglomerular region. | |
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MedLine Citation:
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PMID: 9736284 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Myo-inositol is a major compatible osmolyte in the renal medulla and is accumulated in cells under hypertonic conditions by uptake via a Na+/myo-inositol cotransporter (SMIT). SMIT is regulated by extracellular osmolarity at the transcription level. We investigated localization of SMIT in rat kidney by immunohistochemical staining using an anti-SMIT-antibody raised against a synthetic peptide corresponding to part of SMIT and by in situ hybridization. SMIT protein localized predominantly to the basolateral membranes of cells of the thick ascending limb of Henle (TAL) and inner medullary collecting duct (IMCD). Macula densa (MD) cells, identified as the Tamm-Horsfall-protein (THP)-unreactive cells surrounded by THP-reactive TAL cells, also stained for anti-SMIT. In situ hybridization yielded the intense SMIT signals in the TAL and IMCD and also in the juxtaglomerular (JG) region. Prior loading of the animal with a high concentration of NaCl rapidly induced SMIT mRNA; furosemide down-regulated it. The high level of SMIT expression suggests that MD cells are exposed to hypertonicity at the basolateral surface. Because SMIT expression seemed to be proportional to the magnitude of NaCl reabsorption, it may be a good marker for examination of the tubuloglomerular feedback mechanism in vivo. |
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Authors:
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A Yamauchi; T Sugiura; H Kitamura; Y Matsuoka; E Imai; M Hori |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Kidney international. Supplement Volume: 67 ISSN: 0098-6577 ISO Abbreviation: Kidney Int. Suppl. Publication Date: 1998 Sep |
Date Detail:
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Created Date: 1998-11-23 Completed Date: 1998-11-23 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7508622 Medline TA: Kidney Int Suppl Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: S183-5 Citation Subset: IM |
Affiliation:
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First Department of Medicine, Osaka University School of Medicine, Japan. atsushi@medone.med.osaka-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Autoradiography Carrier Proteins / analysis*, genetics* Gene Expression Glomerular Mesangium / chemistry, metabolism Heat-Shock Proteins / analysis*, genetics* In Situ Hybridization Juxtaglomerular Apparatus / chemistry*, metabolism* Loop of Henle / chemistry, metabolism Male Membrane Proteins* Molecular Sequence Data RNA, Messenger / analysis Rats Rats, Sprague-Dawley Sodium Chloride / metabolism Staining and Labeling Symporters* |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Heat-Shock Proteins; 0/Membrane Proteins; 0/RNA, Messenger; 0/Symporters; 146890-04-2/SLC5A3 protein, human; 7647-14-5/Sodium Chloride |
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