Document Detail

Expression of inflammatory cytokines (interleukin-1 beta and interleukin-6) in amniochorionic membranes.
MedLine Citation:
PMID:  7856675     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: This study was designed to investigate the expression of inflammatory cytokines (interleukin-1 beta and interleukin-6) by fetal membranes in response to infection in vivo and to endotoxin in organ culture. STUDY DESIGN: Amniochorionic membranes were collected from infected and uninfected women and analyzed for cytokine messenger ribonucleic acid and protein. Normal membranes were cultured and exposed to endotoxin. Messenger ribonucleic acid expression was analyzed by reverse transcriptase-polymerase chain reaction. Cellular localization of messenger ribonucleic acid and protein was determined by in situ hybridization and immunocytochemical evaluation, respectively. RESULTS: Messenger ribonucleic acid for interleukin-1 beta appeared to be increased in infected or endotoxin-stimulated amniochorionic membranes, whereas interleukin-6 messenger ribonucleic acid was only observed in infected membranes or after endotoxin stimulation. Interleukin-1 beta messenger ribonucleic acid was localized exclusively to chorionic cells, whereas protein was observed in both chorion and amnion. Interleukin-6 messenger ribonucleic acid and protein were produced in both amniotic and chorionic cells. CONCLUSION: Amniochorionic membranes are a site of inflammatory cytokine production. These findings may have significance in preterm labor or premature rupture of membranes.
R Menon; K F Swan; T W Lyden; N S Rote; S J Fortunato
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  172     ISSN:  0002-9378     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-03-15     Completed Date:  1995-03-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  493-500     Citation Subset:  AIM; IM    
Department of Obstetrics and Gynecology, Tulane University School of Medicine, New Orleans, Louisiana.
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MeSH Terms
Amnion / immunology*
Chorion / immunology*
Culture Techniques
In Situ Hybridization
Interleukin-1 / analysis*,  genetics
Interleukin-6 / analysis*,  genetics
Obstetric Labor, Premature / immunology
Polymerase Chain Reaction
Pregnancy Complications, Infectious / immunology*
RNA, Messenger / analysis
Reg. No./Substance:
0/Endotoxins; 0/Interleukin-1; 0/Interleukin-6; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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