Document Detail


Expression of inducible nitric oxide synthase in failing and non-failing human heart.
MedLine Citation:
PMID:  8745224     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recently, a significant activity of inducible nitric oxide synthase (iNOS) has been reported in biopsies from failing hearts due to idiopathic dilated cardiomyopathy (IDC). Thus, a potential pathophysiological role of iNOS in IDC has been stated. In order to investigate, whether iNOS expression is of pathophysiological relevance in human heart failure, we measured iNOS protein expression and cGMP content in left ventricular myocardium from non-failing and failing human hearts. Immunoblot analysis revealed iNOS protein expression in four out of six failing hearts from septic patients, whereas no iNOS-protein expression was detected in either non-failing human hearts (n = 6) or failing hearts due to IDC (n = 9), ischemic heart disease (IHD, n = 7), Becker muscular dystrophy (BMD, n = 2) and mitoxantrone-induced toxic cardiomyopathy TCM, n = 1). cGMP content was increased by 130% in septic hearts, whereas there was no cGMP increase in hearts with IDC. IHD and BMD compared to non-failing hearts. We conclude, that the induction of iNOS may play a role in contractile dysfunction observed in septic shock, but is unlikely to be of major pathophysiological importance in end-stage heart failure due to IDC, IHD, BMD and TCM.
Authors:
M Thoenes; U Förstermann; W R Tracey; N M Bleese; A K Nüssler; H Scholz; B Stein
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of molecular and cellular cardiology     Volume:  28     ISSN:  0022-2828     ISO Abbreviation:  J. Mol. Cell. Cardiol.     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1996-10-11     Completed Date:  1996-10-11     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0262322     Medline TA:  J Mol Cell Cardiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  165-9     Citation Subset:  IM    
Affiliation:
Abteilung Allgemeine Pharmakologie, Universitäts-Krankenhaus Eppendorf, Hamburg.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cardiomyopathies / chemically induced,  enzymology*,  metabolism
Cardiomyopathy, Dilated / enzymology
Cell Line
Cyclic GMP / metabolism
Gene Expression*
Heart Failure / enzymology*,  etiology
Heart Ventricles
Humans
Isoenzymes / biosynthesis
Macrophages / enzymology
Mice
Mitoxantrone / adverse effects
Muscular Dystrophies / enzymology
Myocardial Ischemia / enzymology
Myocardium / enzymology*,  metabolism
Nitric Oxide Synthase / biosynthesis*
Reference Values
Sepsis / enzymology,  metabolism
Chemical
Reg. No./Substance:
0/Isoenzymes; 65271-80-9/Mitoxantrone; 7665-99-8/Cyclic GMP; EC 1.14.13.39/Nitric Oxide Synthase

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