Document Detail

Expression of inducible nitric oxide synthase in the lower esophageal sphincter of the endotoxemic opossum.
MedLine Citation:
PMID:  12522530     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Endotoxin modulates esophageal motor function by increasing nitric oxide (NO) production. The aims of this study were to examine inducible nitric oxide synthase (iNOS) induction in the lower esophageal sphincter (LES) of endotoxemic opossums and to investigate the effects of aminoguanidine (AG), a selective inhibitor of iNOS, on plasma nitrite/nitrate levels and on iNOS protein and mRNA expression after exposure to lipopolysaccharide (LPS). METHODS: Before and 12 h after the intravenous administration of LPS and/or AG, plasma nitrite/nitrate levels were determined. The iNOS protein and mRNA expression were investigated in the tissues taken from the LES by Western blot and reverse-transcriptase polymerase chain reaction (RT-PCR). RESULTS: Plasma nitrite/nitrate levels were significantly increased by LPS. The increase in plasma nitrite/nitrate produced by LPS was significantly decreased by AG. Western blot and RT-PCR demonstrated that iNOS expression was markedly increased by LPS, and attenuated slightly by AG. CONCLUSIONS: These studies support the hypothesis that endotoxin increases NO production by the induction of iNOS protein and mRNA.
Hyojin Park; Eugene Clark; Joseph J Cullen; John G Koland; Myong Soo Kim; Jeffrey L Conklin
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of gastroenterology     Volume:  37     ISSN:  0944-1174     ISO Abbreviation:  J. Gastroenterol.     Publication Date:  2002  
Date Detail:
Created Date:  2003-01-10     Completed Date:  2003-04-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430794     Medline TA:  J Gastroenterol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1000-4     Citation Subset:  IM    
Department of Internal Medicine, University of Iowa College of Medicine and VA Medical Center, Iowa City, USA.
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MeSH Terms
Base Sequence
Blotting, Western
Disease Models, Animal
Endotoxemia / enzymology*,  physiopathology*
Esophagogastric Junction / drug effects*,  physiopathology*
Gene Expression Regulation
Guanidines / pharmacology*
Molecular Sequence Data
Nitric Oxide / analysis,  metabolism
Nitric Oxide Synthase / genetics,  metabolism*
Nitric Oxide Synthase Type II
RNA, Messenger / analysis
Random Allocation
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Statistics, Nonparametric
Reg. No./Substance:
0/Guanidines; 0/Lipopolysaccharides; 0/RNA, Messenger; 10102-43-9/Nitric Oxide; EC Oxide Synthase; EC Oxide Synthase Type II
Comment In:
J Gastroenterol. 2002;37(12):1090-1   [PMID:  12522546 ]

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