Document Detail

Expression of inducible nitric oxide synthase and nitrotyrosine in borderline leprosy lesions.
MedLine Citation:
PMID:  11736907     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: In the response to T-helper cell (Th1)-type cytokines and interactions with pathogens, high levels of nitric oxide (NO) are produced by activated macrophages expressing the inducible NO synthase (iNOS). The role and importance of reactive nitrogen intermediates (RNIs) such as NO and peroxynitrite in the host response to diseases caused by intracellular pathogens such as Mycobacterium leprae and M. tuberculosis is unclear.
OBJECTIVES: The aim of this study was to investigate the presence of local production of NO and peroxynitrite in borderline leprosy by using antibodies against iNOS and the product of peroxynitrite, nitrotyrosine (NT).
METHODS: We detected the presence of iNOS and NT in skin biopsies from borderline leprosy patients, with and without reversal reaction (RR), by immunohistochemistry (n = 26).
RESULTS: In general, the granulomas from borderline leprosy lesions with and without RR showed high and specific expression of iNOS and NT. Moreover, strong immunoreactivity to iNOS and NT was observed in granulomas surrounding and infiltrating dermal nerves. The expression of iNOS and NT was also strong in keratinocytes, fibroblasts and endothelial cells in close relation to the granulomatous reaction. In contrast, normal human skin showed no expression of iNOS and NT in these cells.
CONCLUSIONS: We conclude that iNOS and NT are expressed in granulomas from borderline leprosy patients with and without RR and propose that RNIs might be involved in the nerve damage following RR in leprosy.
T Schön; R H Hernandez-Pando; Y Negesse; R Leekassa; T Sundqvist; S Britton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The British journal of dermatology     Volume:  145     ISSN:  0007-0963     ISO Abbreviation:  Br. J. Dermatol.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-12-12     Completed Date:  2001-12-27     Revised Date:  2011-10-27    
Medline Journal Info:
Nlm Unique ID:  0004041     Medline TA:  Br J Dermatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  809-15     Citation Subset:  IM    
Department of Medical Microbiology, Faculty of Health Sciences, 581 85 Linköping, Sweden.
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MeSH Terms
Immunoenzyme Techniques
Leprosy, Borderline / enzymology,  metabolism*,  pathology
Leprosy, Tuberculoid / enzymology,  metabolism
Nitric Oxide / biosynthesis
Nitric Oxide Synthase / metabolism*
Nitric Oxide Synthase Type II
Peroxynitrous Acid / biosynthesis
Skin / enzymology,  metabolism
Tyrosine / analogs & derivatives*,  metabolism*
Reg. No./Substance:
10102-43-9/Nitric Oxide; 14691-52-2/Peroxynitrous Acid; 3604-79-3/3-nitrotyrosine; 55520-40-6/Tyrosine; EC protein, human; EC Oxide Synthase; EC Oxide Synthase Type II

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