| Expression and inducibility of CYP1A1, 1A2, 1B1 by β-naphthoflavone and CYP2B22, CYP3As by rifampicin in heart regions and coronary arteries of pig. | |
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MedLine Citation:
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PMID: 22889553 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In this study, the constitutive and inducible expression of the CYP genes (1A1, 1A2, 1B1, 2B22, 3A22, 3A29 and 3A46), related transcriptional factors (AhR, CAR, PXR, and Nrf2) and the antioxidant enzymes SOD, catalase, GSSH-reductase and GSH-peroxidase were investigated in the liver, heart regions and coronary arteries of control pigs and pigs treated with β-naphthoflavone (βNF) or with rifampicin (RIF). Real-time PCR experiments and enzymatic or immunoblot assays showed that CYP1A1 was predominantly enhanced by βNF in a similar manner in all the heart regions, whereas antioxidant enzyme activity was not affected. The rifampicin treatment resulted in an induction of CYP2B22 and CYP3As, at the transcriptional, activity and protein level in liver but not in heart nor in the coronary arteries, despite the expression of CAR and PXR in the cardiac tissues. These results obtained in vivo suggest that pig cardiac tissues may represent a useful model for humans. |
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Authors:
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Andrea Messina; Emanuela Puccinelli; Pier Giovanni Gervasi; Vincenzo Longo |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-10 |
Journal Detail:
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Title: Research in veterinary science Volume: - ISSN: 1532-2661 ISO Abbreviation: Res. Vet. Sci. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0401300 Medline TA: Res Vet Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
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Istituto di Fisiologia Clinica CNR, Area della Ricerca CNR, via Moruzzi, 1, 56100 Pisa, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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