Document Detail


Expression and inducibility of CYP1A1, 1A2, 1B1 by β-naphthoflavone and CYP2B22, CYP3As by rifampicin in heart regions and coronary arteries of pig.
MedLine Citation:
PMID:  22889553     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, the constitutive and inducible expression of the CYP genes (1A1, 1A2, 1B1, 2B22, 3A22, 3A29 and 3A46), related transcriptional factors (AhR, CAR, PXR, and Nrf2) and the antioxidant enzymes SOD, catalase, GSSH-reductase and GSH-peroxidase were investigated in the liver, heart regions and coronary arteries of control pigs and pigs treated with β-naphthoflavone (βNF) or with rifampicin (RIF). Real-time PCR experiments and enzymatic or immunoblot assays showed that CYP1A1 was predominantly enhanced by βNF in a similar manner in all the heart regions, whereas antioxidant enzyme activity was not affected. The rifampicin treatment resulted in an induction of CYP2B22 and CYP3As, at the transcriptional, activity and protein level in liver but not in heart nor in the coronary arteries, despite the expression of CAR and PXR in the cardiac tissues. These results obtained in vivo suggest that pig cardiac tissues may represent a useful model for humans.
Authors:
Andrea Messina; Emanuela Puccinelli; Pier Giovanni Gervasi; Vincenzo Longo
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Publication Detail:
Type:  Journal Article     Date:  2012-08-11
Journal Detail:
Title:  Research in veterinary science     Volume:  94     ISSN:  1532-2661     ISO Abbreviation:  Res. Vet. Sci.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2012-12-25     Completed Date:  2013-06-05     Revised Date:  2014-01-08    
Medline Journal Info:
Nlm Unique ID:  0401300     Medline TA:  Res Vet Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  77-83     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aryl Hydrocarbon Hydroxylases / biosynthesis*
Catalase / biosynthesis
Coronary Vessels / drug effects*,  metabolism
Cytochrome P-450 CYP1A1 / biosynthesis*
Cytochrome P-450 CYP1A2 / biosynthesis*
Cytochrome P-450 CYP3A / biosynthesis*
Cytochrome P-450 Enzyme System / biosynthesis*
Enzyme Induction / drug effects
Heart / drug effects*
Myocardium / metabolism*
Real-Time Polymerase Chain Reaction / veterinary
Rifampin / pharmacology*
Superoxide Dismutase / biosynthesis
Swine / metabolism
beta-Naphthoflavone / pharmacology*
Chemical
Reg. No./Substance:
6051-87-2/beta-Naphthoflavone; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.11.1.6/Catalase; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases; EC 1.14.14.1/Cytochrome P-450 CYP1A1; EC 1.14.14.1/Cytochrome P-450 CYP1A2; EC 1.14.14.1/Cytochrome P-450 CYP3A; EC 1.14.14.1/cytochrome P-450 CYP1B1; EC 1.15.1.1/Superoxide Dismutase; VJT6J7R4TR/Rifampin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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