Document Detail

Expression of immunohistochemical markers for testicular carcinoma in situ by normal human fetal germ cells.
MedLine Citation:
PMID:  7531795     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: It has been hypothesized that carcinoma in situ of the testis (CIS), which is the precursor of invasive testicular germ cell tumours, may arise from fetal germ cells during fetal development rather than later in life. In order to corroborate this hypothesis, we undertook the present study. EXPERIMENTAL DESIGN: Normal human germ cells from 10 first-trimester fetuses and 76 second- and third-trimester testes were investigated for the immunohistochemical expression of the markers of testicular carcinoma in situ. The panel of markers included in the study consisted of placental-like alkaline phosphatase, the protooncogene c-kit protein product, and the antigens for the monoclonal antibodies TRA-1-60 and M2A. The relative numbers of fetal germ cells that demonstrated positive reaction with the markers were calculated. RESULTS: The vast majority of the germ cells (75-100%) in the first-trimester gonads were positive for placental-like alkaline phosphatase, TRA-1-60, and M2A. The c-kit protein was detected in three out of the ten first-trimester gonads. The relative number of germ cells positive for all the markers studied declined rapidly during the first part of the second trimester, and the decrease continued with the fetal age. CONCLUSIONS: The expression of adult carcinoma in situ markers in normal fetal germ cells is consistent with the hypothesis that CIS cells may arise from fetal germ cells, although re-expression of the antigens in postnatally arising CIS cells could provide an alternative explanation. However, we speculate that a transformation of normal fetal germ cells into CIS cells may take place before the end of the 9th week of fetal development. Furthermore, the expression of c-kit in early human fetal germ cells indicates that the c-kit and its ligand play a role in the early human testicular development.
N Jørgensen; E Rajpert-De Meyts; N Graem; J Müller; A Giwercman; N E Skakkebaek
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Laboratory investigation; a journal of technical methods and pathology     Volume:  72     ISSN:  0023-6837     ISO Abbreviation:  Lab. Invest.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-03-10     Completed Date:  1995-03-10     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0376617     Medline TA:  Lab Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  223-31     Citation Subset:  IM    
University Department of Growth and Reproduction, Rigshospitalet, Copenhagen, Denmark.
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MeSH Terms
Alkaline Phosphatase / analysis
Antibodies, Monoclonal / immunology
Carcinoma in Situ / chemistry*,  diagnosis,  embryology
Fetus / chemistry,  cytology*
In Situ Hybridization
Isoenzymes / analysis
Proto-Oncogene Proteins / analysis
Proto-Oncogene Proteins c-kit
Receptor Protein-Tyrosine Kinases / analysis
Receptors, Colony-Stimulating Factor / analysis
Testicular Neoplasms / chemistry*,  diagnosis,  embryology
Testis / chemistry,  cytology*,  embryology,  enzymology
Tumor Markers, Biological / analysis*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Isoenzymes; 0/Proto-Oncogene Proteins; 0/Receptors, Colony-Stimulating Factor; 0/Tumor Markers, Biological; 0/germ-cell AP isoenzyme; EC Proteins c-kit; EC Protein-Tyrosine Kinases; EC Phosphatase

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