| Expression and immunohistochemical localization of Cdc2 and P70S6K in different stages of mouse germ cells. | |
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MedLine Citation:
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PMID: 16329156 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to determine the function and possible relationship between Cdc2 and P(70)S6K, Western blot analysis and immunohistochemistry analysis were used to study the expression and kinase activity of Cdc2 and P(70)S6K in male mouse germ cells. With the maturation of germ cells in the testis, the expression of Cdc2 and P(70)S6K was relatively constant. However, the kinase activity of P(70)S6K was increased and the phosphorylation of Tyr15 residue of Cdc2 was enhanced, which suggests that the kinase activity of Cdc2 is decreasing. Immunohistochemistry analysis also showed that there was a P(70)S6K transfer from nucleus to cytoplasm during spermatogenesis. During spermatogenesis, cell division of the germ cell in male mouse is decelerated; nevertheless, cell growth is enhanced. Cdc2 and P(70)S6K are involved in these two processes. It could be an alternative mechanism to prepare for future fertilization that Cdc2 is able to maintain a subtle balance between the production and growth of male germ cells by regulating P(70)S6K. |
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Authors:
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Bing-Zhi Yu; Yu-Tong Song; Da-Hai Yu; Wen-Hui Su; Venuste Gasana; Yan-Xiao Li; Zhe Zhang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cell biochemistry and function Volume: 24 ISSN: 0263-6484 ISO Abbreviation: Cell Biochem. Funct. Publication Date: 2006 Mar-Apr |
Date Detail:
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Created Date: 2006-03-06 Completed Date: 2006-05-25 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8305874 Medline TA: Cell Biochem Funct Country: England |
Other Details:
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Languages: eng Pagination: 113-7 Citation Subset: IM |
Copyright Information:
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Copyright 2005 John Wiley & Sons, Ltd. |
Affiliation:
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Department of Biochemistry, China Medical University, Shenyang, People's Republic of China. ybzbiochem@yeah.net |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western CDC2 Protein Kinase / analysis, biosynthesis* Gene Expression Regulation, Developmental Immunohistochemistry Male Maturation-Promoting Factor / physiology Mice Ribosomal Protein S6 Kinases, 70-kDa / analysis, biosynthesis* Spermatocytes / enzymology Spermatozoa / enzymology* |
| Chemical | |
Reg. No./Substance:
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EC 2.7.11.1/Ribosomal Protein S6 Kinases, 70-kDa; EC 2.7.11.22/CDC2 Protein Kinase; EC 2.7.11.22/Maturation-Promoting Factor |
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