Document Detail

Expression of immediate early gene proteins following axotomy and inhibition of axonal transport in the rat central nervous system.
MedLine Citation:
PMID:  8278059     Owner:  NLM     Status:  MEDLINE    
The expression of the immediate early gene-encoded proteins c-Jun, Jun B, Jun D, c-Fos, Fos B and Krox-24 in central neurons following transection of, or inhibition of, axonal transport in their axons was investigated in the rat using immunocytochemistry. Transection of the medial forebrain bundle, which produces an essentially complete axotomy of neurons in the ipsilateral mammillary nucleus, substantia nigra pars compacta, ventral tegmental area and parafascicularis, induced the expression of c-Jun, Jun D and, to a lesser extent, Krox-24, in these nuclei. Microinjection of colchicine into the medial forebrain bundle to chemically inhibit axonal transport similarly induced the expression of these proteins in these areas. The expression of the proteins was first evident 24 h after transection, reached a maximum at 48 h and was still present after 10 days. However, after 30 days the proteins were absent from the substantia nigra, ventral tegmentum and parafascicularis, and were still present only in the mammillary nuclei. The other immediate early genes, Jun B, c-Fos and Fos B, were never expressed above the basal levels seen in untreated rats. Transection of the corpus callosum and the hippocampal commissure, which produces only a partial axotomy of neurons in the cerebral cortex and hippocampus, respectively, did not induce the expression of any of the genes in these neurons. Microinjection of colchicine or vinblastine to produce a localized inhibition of axonal transport in the cerebral cortex, hippocampus, thalamus and cerebellum also induced the expression of c-Jun, Jun D and, again to a lesser extent, Krox-24, in neurons surrounding the injection site. In contrast to this selective expression, administration of the neuronal excitant metrazole induced the expression of all six immediate early gene proteins in central nervous system neurons. These results demonstrate that transection of, or inhibition of, transport in the axons of central neurons induces a particular pattern of expression of transcriptionally operating immediate early genes that may be related to the regenerative competency of the neurons.
J D Leah; T Herdegen; A Murashov; M Dragunow; R Bravo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience     Volume:  57     ISSN:  0306-4522     ISO Abbreviation:  Neuroscience     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1994-02-09     Completed Date:  1994-02-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  53-66     Citation Subset:  IM    
School of Science, Griffith University, Nathan, Australia.
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MeSH Terms
Axons / metabolism,  physiology*
Biological Transport
Brain / metabolism,  physiology*
Colchicine / pharmacology
Corpus Callosum / physiology
Gene Expression* / drug effects
Genes, Immediate-Early*
Hippocampus / physiology
Mamillary Bodies / physiology
Medial Forebrain Bundle / physiology
Rats, Wistar
Vinblastine / pharmacology
Reg. No./Substance:
64-86-8/Colchicine; 865-21-4/Vinblastine

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