Document Detail

Expression of iNOS mRNA associated with suppression of colonic contraction in rat colitis.
MedLine Citation:
PMID:  16866779     Owner:  NLM     Status:  MEDLINE    
AIM: Nitric oxide (NO) synthesis and inducible NO synthase (NOS) expression are increased in colon of patients with inflammatory bowel disease (IBD) and associated with decreased contractility. The aim was to investigate which subtype of NOS that is activated in experimental colitis. METHODS: Experimental colitis was induced in Sprague-Dawley rats by Escherichia coli endotoxin. Expression of different subtypes of NOS was compared in normal and inflamed colon using reverse transcriptase-polymerase chain reaction. In organ baths, isometric contractile responses to acetylcholine (ACh) were studied in the colon, before and after incubation with the NOS inhibitor; N(omega)-nitro-L-arginine methyl ester (L-NAME) and NO donor glyceryl trinitrate. RESULTS: Inflammation decreased colonic contraction to ACh from a pD(2) value of 7.09 +/- 0.16 to 5.30 +/- 0.17 (P < 0.001), and reduced maximal response to ACh. Pre-treatment with L-NAME reversed contractility and shifted the pD(2) for ACh from 5.30 +/- 0.17 to 6.60 +/- 0.19 (P < 0.001) along with a normalized contraction efficacy. RT-PCR product of iNOS was obtained only in rats treated with endotoxin. CONCLUSION: Expression of iNOS is increased in inflamed colonic tissue. The induced overproduction of NO is likely to be responsible for the decreased motility in colitis where NO is suggested to exert a suppressive tone on colonic contractility, which is reversed by blockade of the enzyme.
S Lundberg; M Holst; P M Hellström
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta physiologica (Oxford, England)     Volume:  187     ISSN:  1748-1708     ISO Abbreviation:  Acta Physiol (Oxf)     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-07-26     Completed Date:  2007-03-29     Revised Date:  2009-02-03    
Medline Journal Info:
Nlm Unique ID:  101262545     Medline TA:  Acta Physiol (Oxf)     Country:  England    
Other Details:
Languages:  eng     Pagination:  489-94     Citation Subset:  IM    
Department of Medicine, Unit of Gastroenterology and Hepatology, Karolinska University Hospital Solna, Karolinska Institutet, Stockholm, Sweden.
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MeSH Terms
Acetylcholine / pharmacology
Colitis / enzymology,  etiology,  physiopathology*
Colon / drug effects,  physiopathology
Gastrointestinal Motility*
Gene Expression
Muscle Contraction / drug effects
Muscle, Smooth / drug effects,  physiopathology
Nitric Oxide / biosynthesis
Nitric Oxide Synthase Type II / biosynthesis,  genetics,  physiology*
RNA, Messenger / genetics
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction / methods
Tissue Culture Techniques
Vasodilator Agents / pharmacology
Reg. No./Substance:
0/Endotoxins; 0/RNA, Messenger; 0/Vasodilator Agents; 10102-43-9/Nitric Oxide; 51-84-3/Acetylcholine; 67924-63-4/endotoxin, Escherichia coli; EC Oxide Synthase Type II

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