| Expression of human recombinant plasminogen activators enhances invasion and experimental metastasis of H-ras-transformed NIH 3T3 cells. | |
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MedLine Citation:
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PMID: 2747645 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The gene transfer technique was used to examine the role of plasminogen activator (PA) in the invasive and metastatic behavior of tumorigenic cells. H-ras-transformed NIH 3T3 clonal cells producing a very low level of PA were generated and further transfected with an expression plasmid containing a cDNA sequence encoding either the urokinase-type or the tissue-type human PA. Compared with the parental transformed cells, clonal cells expressing high levels of both types of recombinant PA invaded more rapidly through a basement membrane reconstituted in vitro. Furthermore, cells expressing high levels of recombinant urokinase-type PA also caused a higher incidence of pulmonary metastatic lesions after intravenous injection into nude mice. Both activities were reduced by the serine proteinase inhibitor EACA; invasion was also suppressed by antibodies blocking the activity of human PAs and by the synthetic collagenase inhibitor SC-44463. These findings provide direct genetic evidence for a causal role of PA in invasive and metastatic activities. |
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Authors:
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J H Axelrod; R Reich; R Miskin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular and cellular biology Volume: 9 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1989 May |
Date Detail:
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Created Date: 1989-08-18 Completed Date: 1989-08-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2133-41 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Basement Membrane / physiology Cell Transformation, Neoplastic* Gene Expression Regulation Humans Mice Neoplasm Invasiveness Neoplasm Metastasis Plasminogen Activators / biosynthesis, genetics* Proto-Oncogenes |
| Chemical | |
Reg. No./Substance:
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EC 3.4.21.-/Plasminogen Activators |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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