Document Detail


Expression of human immunodeficiency virus (HIV) in naturally infected peripheral blood mononuclear cells: comparison of a standard co-culture technique with a newly developed microculture method.
MedLine Citation:
PMID:  1909827     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Peripheral blood mononuclear cells (PBMCs) from 29 patients infected with human immunodeficiency virus (HIV) were cultured by two different methods. One was the standard co-culture technique, the other a newly developed microculture method. In this assay 10(6) PBMCs were cultivated in 250 microliters medium, no activating agents or allogeneic cells were present. P24 antigen production measured by this method was found in 7 out of 11 PBMC cultures of patients in the Walter Reed (WR) stage 1 or 2, whereas only 4 samples were positive by the co-culture procedure. Cultures from patients in the later stages of the disease (WR 5/6) showed a higher p24 production by the co-culture method than by the microculture assay. It is assumed that rapidly growing HIV strains can be better assessed by the co-culture method which may select for these strains. P24 expression can be more easily obtained by the microculture technique even in cases where slowly replicating strains may be present. In conclusion, results from the microculture procedure described may be a useful supplementation to findings observed by the co-culture method.
Authors:
B Eberlein; A Baur; M Neundorfer; G Jahn
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virus research     Volume:  19     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-10-11     Completed Date:  1991-10-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  153-61     Citation Subset:  IM; X    
Affiliation:
Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, F.R.G.
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MeSH Terms
Descriptor/Qualifier:
Adult
Female
Gene Products, gag / biosynthesis
HIV Antigens / biosynthesis
HIV Core Protein p24
HIV Infections / microbiology
HIV-1 / immunology,  physiology*
Humans
Leukocytes, Mononuclear / microbiology*
Male
Viral Core Proteins / biosynthesis
Virus Cultivation / methods*
Virus Replication
Chemical
Reg. No./Substance:
0/Gene Products, gag; 0/HIV Antigens; 0/HIV Core Protein p24; 0/Viral Core Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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