| Expression of human ferredoxin in Saccharomyces cerevisiae: mitochondrial import of the protein and assembly of the [2Fe-2S] center. | |
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MedLine Citation:
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PMID: 1314545 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vertebrate ferredoxins function in the transfer of reducing equivalents from NADPH:ferredoxin oxidoreductase to cytochrome P450 enzymes involved in steroid metabolism. We report here the expression of human mitochondrial ferredoxin in the yeast Saccharomyces cerevisiae. The full-length ferredoxin protein containing the ferredoxin mitochondrial leader sequence could not be stably expressed in S. cerevisiae, but a fusion protein consisting of the mature portion of ferredoxin linked to the mitochondrial leader sequence of the S. cerevisiae cytochrome c oxidase subunit Va protein (COX5a) could be stably expressed. The COX5a:ferredoxin fusion protein was targeted to the mitochondria as a preprotein and was cleaved at the normal processing site of the COX5a presequence during import into the matrix. Absorption spectra and electron transfer activity of the isolated fusion protein established that the [2Fe-2S] center was correctly assembled and incorporated into the recombinant ferredoxin in this heterologous system. |
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Authors:
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B L Seaton; L E Vickery |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 294 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 1992 May |
Date Detail:
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Created Date: 1992-05-15 Completed Date: 1992-05-15 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 603-8 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, University of California, Irvine 92717. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Electron Transport Complex IV / biosynthesis, genetics*, isolation & purification Electrophoresis, Polyacrylamide Gel Ferredoxins / biosynthesis*, genetics, isolation & purification Genes, Fungal Humans Kinetics Mitochondria / metabolism* Molecular Sequence Data Molecular Weight Oligodeoxyribonucleotides Plasmids Promoter Regions, Genetic Protein Sorting Signals / genetics Recombinant Fusion Proteins / biosynthesis*, isolation & purification Saccharomyces cerevisiae / genetics*, metabolism Spectrophotometry |
| Grant Support | |
ID/Acronym/Agency:
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GM43548/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ferredoxins; 0/Oligodeoxyribonucleotides; 0/Protein Sorting Signals; 0/Recombinant Fusion Proteins; EC 1.9.3.1/Electron Transport Complex IV |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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