Document Detail


Expression of the high mobility group A family member p8 is essential to maintaining tumorigenic potential by promoting cell cycle dysregulation in LbetaT2 cells.
MedLine Citation:
PMID:  17451874     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mechanism by which the HMGA protein p8 facilitates tumorigenesis may be cell cycle dysregulation. Control- (C) LbetaT2 cells, which express p8, form tumors at a rate five-times faster than p8-knockdown (p8-KD)-LbetaT2 cells. In association with this heightened tumorigenic potential, p8-expressing C-LbetaT2 cells avoid G(0)/G(1) arrest and become genetically unstable while p8-KD-LbetaT2 cells arrest in G(0)/G(1), become senescent upon overgrowth, and maintain a diploid population. These phenotypic changes correspond to altered cell cycle regulation at the G(1)-to-S transition that may be due to p8-mediated changes in expression of the Cip/Kip family members of cell cycle inhibitors, p21, p27, and p57.
Authors:
K M Brannon; C M Million Passe; C R White; N A Bade; M W King; C C Quirk
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-23
Journal Detail:
Title:  Cancer letters     Volume:  254     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-30     Completed Date:  2007-09-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  146-55     Citation Subset:  IM    
Affiliation:
Department of Biology, Indiana University, Bloomington, IN 47405-4401, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Cell Aging / genetics,  physiology
Cell Cycle / genetics*,  physiology
Cell Line, Transformed
Cell Proliferation
Cyclin-Dependent Kinase Inhibitor p21 / genetics,  metabolism
Cyclin-Dependent Kinase Inhibitor p27 / genetics,  metabolism
Cyclin-Dependent Kinase Inhibitor p57 / genetics,  metabolism
DNA-Binding Proteins / genetics*,  metabolism
G0 Phase / genetics,  physiology
G1 Phase / genetics,  physiology
Gene Expression
HMGA Proteins / genetics,  metabolism
Mice
Mice, Nude
Mutation
Neoplasm Proteins / genetics*,  metabolism
Neoplasms, Experimental / genetics,  metabolism,  pathology*
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
beta-Galactosidase / metabolism
Chemical
Reg. No./Substance:
0/Cdkn1a protein, mouse; 0/Cdkn1b protein, mouse; 0/Cdkn1c protein, mouse; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclin-Dependent Kinase Inhibitor p57; 0/DNA-Binding Proteins; 0/HMGA Proteins; 0/Neoplasm Proteins; 0/Nupr1 protein, mouse; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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