Document Detail


Expression of hemoglobin in rodent neurons.
MedLine Citation:
PMID:  19116637     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hemoglobin is the major protein in red blood cells and transports oxygen from the lungs to oxygen-demanding tissues, like the brain. Mechanisms that facilitate the uptake of oxygen in the vertebrate brain are unknown. In invertebrates, neuronal hemoglobin serves as intracellular storage molecule for oxygen. Here, we show by immunohistochemistry that hemoglobin is specifically expressed in neurons of the cortex, hippocampus, and cerebellum of the rodent brain, but not in astrocytes and oligodendrocytes. The neuronal hemoglobin distribution is distinct from the neuroglobin expression pattern on both cellular and subcellular levels. Probing for low oxygen levels in the tissue, we provide evidence that hemoglobin alpha-positive cells in direct neighborhood with hemoglobin alpha-negative cells display a better oxygenation than their neighbors and can be sharply distinguished from those. Neuronal hemoglobin expression is upregulated by injection or transgenic overexpression of erythropoietin and is accompanied by enhanced brain oxygenation under physiologic and hypoxic conditions. Thus we provide a novel mechanism for the neuroprotective actions of erythropoietin under ischemic-hypoxic conditions. We propose that neuronal hemoglobin expression is connected to facilitated oxygen uptake in neurons, and hemoglobin might serve as oxygen capacitator molecule.
Authors:
Dominik W Schelshorn; Armin Schneider; Wolfgang Kuschinsky; Daniela Weber; Carola Krüger; Tanjew Dittgen; Heinrich F Bürgers; Fatemeh Sabouri; Nikolaus Gassler; Alfred Bach; Martin H Maurer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-31
Journal Detail:
Title:  Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     Volume:  29     ISSN:  1559-7016     ISO Abbreviation:  J. Cereb. Blood Flow Metab.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-02-27     Completed Date:  2009-03-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8112566     Medline TA:  J Cereb Blood Flow Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  585-95     Citation Subset:  IM    
Affiliation:
Division of Systems Physiology, Department of Physiology and Pathophysiology, University of Heidelberg, Heidelberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / pharmacology
Cell Hypoxia
Cells, Cultured
Cerebellum / cytology,  drug effects,  metabolism*
Cerebral Cortex / cytology,  drug effects,  metabolism*
Electrophoresis, Gel, Two-Dimensional
Erythropoietin, Recombinant / genetics,  pharmacology
Female
Hemoglobins / biosynthesis*
Hippocampus / cytology,  drug effects,  metabolism*
Mass Spectrometry
Mice
Mice, Inbred C57BL
Mice, Transgenic
Neurons / drug effects,  metabolism*
Nitroimidazoles / pharmacology
Oxygen / metabolism
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Species Specificity
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Erythropoietin, Recombinant; 0/Hemoglobins; 0/Nitroimidazoles; 70132-51-3/pimonidazole; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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