Document Detail

Expression of glucose transporter 1 in adult and developing human peripheral nerve.
MedLine Citation:
PMID:  7681417     Owner:  NLM     Status:  MEDLINE    
Northern hybridization of total RNA isolated from adult human sciatic nerve demonstrated a readily detectable hybridization signal for glucose transporter 1 (GLUT 1) mRNA. Western blot analysis demonstrated that GLUT 1 proteins extracted from adult human and from mature rat sciatic nerves had different electrophoretical mobilities. The migration positions of human and rat GLUT 1 proteins corresponded to 60-70 kDa and 55-60 kDa, respectively, as estimated by markers with known molecular masses. Indirect immunofluorescence staining localized GLUT 1 to the perineurium in the adult human sciatic nerve. Only a few endoneurial capillaries of human adult nerve stained positively for GLUT 1, which was in contrast to rat peripheral nerve where most endoneurial capillaries were positive for GLUT 1. In developing human nerves, the staining pattern for GLUT 1 was markedly different from that of the adult nerves: at 14 weeks, the perineurial cells were entirely negative for GLUT 1. Between 22 and 26 weeks of gestation, the staining reaction for GLUT 1 in the perineurium became markedly more prominent, and by 35 weeks the intense perineurial staining resembled that observed in the adult human nerves. In contrast to adult nerves, both endo- and epineurial blood vessels stained intensely for GLUT 1 in the fetal samples. Thus, the immunoreactivity for GLUT 1 in the perineurium seems to increase concomitant with the maturation of barrier properties of perineurium, whereas the transient expression of GLUT 1 in the vasculature of developing nerve may have a specific role in the proliferating endothelial cells.
P Muona; S Jaakkola; V Salonen; J Peltonen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diabetologia     Volume:  36     ISSN:  0012-186X     ISO Abbreviation:  Diabetologia     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-04-27     Completed Date:  1993-04-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006777     Medline TA:  Diabetologia     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  133-40     Citation Subset:  IM    
Department of Medical Biochemistry, University of Turku, Finland.
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MeSH Terms
Blotting, Northern
Blotting, Western
Cell Differentiation / physiology
Cell Division / physiology
Endothelium, Vascular / chemistry,  cytology
Epitopes / analysis
Fetus / innervation
Fluorescent Antibody Technique
Glucose Transporter Type 1
Monosaccharide Transport Proteins / analysis*,  genetics,  physiology
Peripheral Nerves / chemistry*,  embryology
RNA, Messenger / analysis,  genetics
Sciatic Nerve / chemistry,  embryology
Reg. No./Substance:
0/Epitopes; 0/Glucose Transporter Type 1; 0/Monosaccharide Transport Proteins; 0/RNA, Messenger; 0/SLC2A1 protein, human

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