Document Detail


Expression and genomic structure of the dormancy-associated MADS box genes MADS13 in Japanese pears (Pyrus pyrifolia Nakai) that differ in their chilling requirement for endodormancy release.
MedLine Citation:
PMID:  23761324     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We isolated three dormancy-associated MADS-box (DAM) genes (MADS13-1, MADS13-2 and MADS13-3) and showed regulated expression concomitant with endodormancy establishment and release in the leaf buds of Japanese pear 'Kosui'. Comparative analysis between 'Kosui' and Taiwanese pear TP-85-119 ('Hengshanli'), a less dormant pear cultivar, showed reduction of MADS13-1 expression level in 'Hengshanli' earlier than in 'Kosui' towards endodormancy release, suggesting the possible relationship between chilling requirement and MADS13-1 expression. Application of hydrogen cyanamide accelerated endodormancy release with a reduction in MADS13 expression, whereas heat treatment in autumn inhibited endodormancy establishment without induction of MADS13 expression, indicating a close relationship between the MADS13 expression pattern and endodormancy phase transitions. Moreover, both the cis-acting regulatory elements and the methylation status in the 5' upstream region of the MADS13-1 gene were not largely different between 'Kosui' and 'Hengshanli'. Genomic structures of MADS13-1 from 'Kosui' and 'Hengshanli' revealed a 3218 bp insertion in the first intron of 'Hengshanli' that might be ascribed to the lower expression of MADS13-1tw; however, this insertion was also found in pear genotypes with a high chilling requirement. These results indicated that the low expression of MADS13-1 in 'Hengshanli' towards endodormancy release could not be explained by the identified cis-acting regulatory elements, the methylation status of the putative promoter or by intron insertion.
Authors:
Takanori Saito; Songling Bai; Akiko Ito; Daisuke Sakamoto; Toshihiro Saito; Banjamin Ewa Ubi; Tsuyoshi Imai; Takaya Moriguchi
Related Documents :
23756994 - Classification and evolutionary analysis of the basic helix-loop-helix gene family in t...
23850284 - Draft assembly of the symbiodinium minutum nuclear genome reveals dinoflagellate gene s...
24747724 - Influence of crop load on the expression patterns of starch metabolism genes in alterna...
23941144 - Targeted disruption of transcriptional regulators in chaetomium globosum activates bios...
23159244 - Deletion of invh gene in salmonella enterica serovar typhimurium limits the secretion o...
17196704 - Prognostic significance of rassf1a promoter methylation on survival of non-small cell l...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-6-10
Journal Detail:
Title:  Tree physiology     Volume:  -     ISSN:  1758-4469     ISO Abbreviation:  Tree Physiol.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-6-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100955338     Medline TA:  Tree Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Comprehensive genetic analysis identifies a pathognomonic NAB2/STAT6 fusion gene, nonrandom secondar...
Next Document:  How to use: the neonatal neurological examination.