Document Detail


Expression of genes involved in calcium absorption in human duodenum.
MedLine Citation:
PMID:  10202378     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The role of vitamin D metabolites in the regulation of expression of genes involved in dietary calcium absorption by the human intestine remains poorly understood despite much work in animals. MATERIALS AND METHODS: To investigate this, we measured the expression of transcripts for two of these genes, calbindin-D9k and the basolateral membrane calcium pumping ATPase, PMCA1, in duodenal endoscopic biopsies from 40 subjects. Northern blots were hybridized with previously characterized probes, and the signal was quantified by phosphor imaging. These transcript levels were related to circulating vitamin D metabolites and also analysed according to the vitamin D receptor gene TaqI polymorphism determined in DNA from blood. RESULTS: Plasma 1,25-dihydroxycholecalciferol correlated significantly with calbindin-D9k RNA (r = 0.4, P < 0.02) but not with PMCA1. Plasma 25-OH-cholecalciferol was not correlated with transcripts for either gene and, furthermore, the mean levels of these transcripts did not differ significantly when grouped by vitamin D receptor genotype. CONCLUSION: In normal humans, 1, 25-dihydroxycholecalciferol has a small but significant relationship to duodenal expression of calbindin-D9k, but not to PMCA1 expression.
Authors:
J R Walters; A Howard; L J Lowery; E B Mawer; S Legon
Related Documents :
24880148 - The nucleosome remodeling and deacetylase complex in development and disease.
25101798 - High-density genotyping of immune loci in kawasaki disease and ivig treatment response ...
10794598 - Association between angiotensin-converting enzyme gene polymorphism and coronary artery...
19593948 - Elastin gene study of infants with isolated congenital ductus arteriosus aneurysm.
21080078 - Characterization of ssu5c promoter of a rbcs gene from duckweed (lemna gibba).
16932148 - Contact-call driven and tone-driven zenk expression in the nucleus ovoidalis of the bud...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of clinical investigation     Volume:  29     ISSN:  0014-2972     ISO Abbreviation:  Eur. J. Clin. Invest.     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-04-29     Completed Date:  1999-04-29     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  0245331     Medline TA:  Eur J Clin Invest     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  214-9     Citation Subset:  IM    
Affiliation:
Imperial College School of Medicine, Hammersmith Hospital, London. jwalters@rpms.ac.uk
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Calcitriol / blood
Calcium / metabolism*
Calcium-Binding Protein, Vitamin D-Dependent / genetics
Duodenum / metabolism*
Gene Expression Regulation*
Genotype
Humans
Intestinal Absorption / genetics*
Receptors, Calcitriol / genetics
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Receptors, Calcitriol; 0/calbindin; 32222-06-3/Calcitriol; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Post-prandial chylomicron response may be predicted by a single measurement of plasma apolipoprotein...
Next Document:  Dynamics of reactive oxygen intermediate production in human glioma: n-6 essential fatty acid effect...