Document Detail

Expression of genes involved in calcium absorption in human duodenum.
MedLine Citation:
PMID:  10202378     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The role of vitamin D metabolites in the regulation of expression of genes involved in dietary calcium absorption by the human intestine remains poorly understood despite much work in animals. MATERIALS AND METHODS: To investigate this, we measured the expression of transcripts for two of these genes, calbindin-D9k and the basolateral membrane calcium pumping ATPase, PMCA1, in duodenal endoscopic biopsies from 40 subjects. Northern blots were hybridized with previously characterized probes, and the signal was quantified by phosphor imaging. These transcript levels were related to circulating vitamin D metabolites and also analysed according to the vitamin D receptor gene TaqI polymorphism determined in DNA from blood. RESULTS: Plasma 1,25-dihydroxycholecalciferol correlated significantly with calbindin-D9k RNA (r = 0.4, P < 0.02) but not with PMCA1. Plasma 25-OH-cholecalciferol was not correlated with transcripts for either gene and, furthermore, the mean levels of these transcripts did not differ significantly when grouped by vitamin D receptor genotype. CONCLUSION: In normal humans, 1, 25-dihydroxycholecalciferol has a small but significant relationship to duodenal expression of calbindin-D9k, but not to PMCA1 expression.
J R Walters; A Howard; L J Lowery; E B Mawer; S Legon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of clinical investigation     Volume:  29     ISSN:  0014-2972     ISO Abbreviation:  Eur. J. Clin. Invest.     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-04-29     Completed Date:  1999-04-29     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  0245331     Medline TA:  Eur J Clin Invest     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  214-9     Citation Subset:  IM    
Imperial College School of Medicine, Hammersmith Hospital, London.
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MeSH Terms
Calcitriol / blood
Calcium / metabolism*
Calcium-Binding Protein, Vitamin D-Dependent / genetics
Duodenum / metabolism*
Gene Expression Regulation*
Intestinal Absorption / genetics*
Receptors, Calcitriol / genetics
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Receptors, Calcitriol; 0/calbindin; 32222-06-3/Calcitriol; 7440-70-2/Calcium

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