| Expression of gamma-synuclein in colorectal cancer tissues and its role on colorectal cancer cell line HCT116. | |
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MedLine Citation:
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PMID: 19859996 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To investigate the expression pattern of gamma-synuclein in colorectal cancer (CRC) tissues, and to study the effects of gamma-synuclein on CRC cell line HCT116 biological features in vitro. METHODS: The expression pattern of gamma-synuclein was determined in 54 CRC tissues and 30 tumor-matched nonneoplastic adjacent tissues (NNAT) 5 cm away from the tumor via real-time quantitative reverse transcription PCR (RT-PCR) and immunohistochemistry. The relationship between gamma-synuclein protein expression and clinicopathological factors of CRC tissues was analyzed. Three small interfering RNA (siRNA) targeting gamma-synuclein mRNA plasmids were constructed and transfected into the CRC cell line HCT116. The stable cell lines were selected with G-418 for 28 d, and the biological features of these cells were examined by cell growth curve, soft agar assay, and cell migration and invasion assays in vitro. RESULTS: The expression of gamma-synuclein mRNA and protein was much higher in CRC tissue samples than in NNAT samples (P = 0.02, P = 0.036). There was a significant correlation between the gamma-synuclein protein expression and clinical stage and lymph node involvement of CRC (P = 0.02, P = 0.033). In functional analysis we found that down-regulation of gamma-synuclein expression in HCT116 cells could inhibit the growth, colony formation rate, and migration and invasion ability of HCT116 cells. CONCLUSION: Increased expression of gamma-synuclein in CRC tissues and the biological effects of reduced gamma-synuclein expression on HCT116 cells suggest that gamma-synuclein may play a positive role in the progression of CRC. |
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Authors:
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Qing Ye; Bo Feng; Yuan-Fei Peng; Xue-Hua Chen; Qu Cai; Bei-Qin Yu; Liang-Hui Li; Ming-Yuan Qiu; Bing-Ya Liu; Min-Hua Zheng |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 15 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-10-27 Completed Date: 2010-01-12 Revised Date: 2010-09-28 |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 5035-43 Citation Subset: IM |
Affiliation:
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Shanghai Institute of Digestive Surgery, No. 197 Ruijin Second Road, Shanghai 200025, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line, Tumor Cell Movement Cell Proliferation Colorectal Neoplasms / metabolism* Disease Progression Gene Expression Profiling Gene Expression Regulation, Neoplastic* Humans Immunohistochemistry / methods Neoplasm Invasiveness RNA, Messenger / metabolism RNA, Small Interfering / metabolism Reverse Transcriptase Polymerase Chain Reaction Time Factors gamma-Synuclein / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/RNA, Small Interfering; 0/gamma-Synuclein |
| Comments/Corrections | |
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